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2100 Bioanalyzer - RNA Solutions

Industry standard of RNA quality control
Due to the omnipresence of RNases, and the instability of RNA, integrity checks and sample quantitation are essential steps before any RNA dependent application. Microfluidics technology makes it easier and faster to analyze samples using just 1 µL of a RNA sample. Agilent offers the RNA Nano 6000 and the RNA Pico 6000 kits for this analysis. In addition the 2100 Bioanalyzer Expert software generates the RNA Integrity Number(RIN), a quantitation estimate, and will calculate ribosomal ratios of the total RNA sample. For mRNA samples the percentage of ribosomal impurities is calculated. RIN can be used as a standard integrity measure that is independent of concentration, instrument or analyst.

  2100 Bioanalyzer Electrophoresis Agilent
 
  • Minimal sample consumption – use as little as 5 ng of  total RNA or 200 pg of total RNA for analysis, saving most of your valuable preps?
  • Faster results – analyze 11 to 12 samples in 30 minutes
  • RNA Integrity Number (RIN) – a reliable tool to compare  integrity of RNA samples automatically  plus freely accessible
  • RNA Integrity Database (RINdb) of RNA profiles
  • Alternative data display options – results shown in gel-like image,  electropherogram and tabular formats
  • RNase free – use RNase free reagents and chips to avoid  sample degradation during analysis
  • Ready-to-use - standardized assays and pre-packaged reagent kits
For the full list of available part numbers for the 2100 Bioanalyzer please click here
For the analysis of small RNA please click here
Part Numbers and Prices
 
Description Part Number
Agilent DNA 1200 Reagents (Reagents for Separation Only)
5067-1509
Agilent RNA 6000 Nano Kit (includes chips)
5067-1511
Agilent RNA 6000 Nano Reagents (Reagents for Separation Only)
5067-1512
Agilent RNA 6000 Pico Kit (includes chips)
5067-1513
Agilent RNA 6000 Pico Reagents (Reagents for Separation Only)
5067-1514
Agilent 2100 Small RNA Kit (Chip Kit)
5067-1548
Agilent Small RNA Reagents (Reagents for Separation Only)
5067-1549
Agilent Small RNA Ladder 10µl at 10X
5067-1550
 
 
 
RNA Integrity Number (RIN)
 

Overview

The RNA integrity number (RIN) is a software tool designed to help scientists estimate the integrity of total RNA samples. The expert software automatically assigns an integrity number to an eukaryote total RNA sample.Using this tool, sample integrity is no longer determined by the ratio of the ribosomal bands, but by the entire electrophoretic trace of the RNA sample. This includes the presence or absence of degradation products. In this way, interpretation of an electropherogram is facilitated, comparison of samples is enabled and repeatability of experiments is ensured.
The assigned RIN is independent of sample concentration, instrument and analyst therefore becoming a de facto standard for RNA integrity.
The RNA Integrity Database (RINdb) is a freely accessible repository holding hundreds of user submitted total RNA traces. By searching the database scientists can now see what is a “normal” profile for different tissue types as well as the effects of using different RNA extraction methods and kits. It is important to understand what the RIN can and cannot do.

What the RIN can do:

  • Obtain a numerical assessment of the integrity of RNA.
  • Directly compare RNA samples, e.g. before and after archival, compare integrity of same tissue across different labs.
  • Ensure repeatability of experiments, e.g. if RIN shows a given value and is suitable for microarray experiments, then the RIN of the same value can always be used for similar experiments given that the same organism/tissue/extraction method is used.

What the RIN cannot do:

  • Tell a scientist ahead of time whether an experiment will work or not if no prior validation was done (e.g. RIN of 5 might not work for microarray experiments, but might work well for an appropriate RT-PCR experiment. Also, a RIN that might be good for a 3' amplification might not work for a 5' amplification). Click here for a proposed use model to achieve best results.

Scientific Journals

  • Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, Lightfoot S, Menzel W, Granzow M and Ragg T. The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Molecular Biology 2006, 7:3 (31 Jan 2006)
  • Imbeaud S, Graudens E, Boulanger V, Barlet X, Zaborski P, Eveno E, Mueller O, Schroeder A, and Auffray C. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces, Nucl. Acids Res. 2005 33: e56.
  • Tissue Microarrays Reach New Markets

Agilent Publications

 

 


 
 
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