Agilent Bio SEC-5 HPLC Columns
		Highest resolution size-based separations for biological molecules Agilent Bio SEC-5 HPLC columns are packed with 5 μm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution. Agilent Bio SEC-5 HPLC columns are available in 100Å, 150Å, 300Å, 500Å, 1000Å and 2000Å.
Maximum recovery for a broad range of size-based, bio-molecule separations Outstanding reproducibility and column lifetime Excellent stability, even under high-pH, high-salt, and low-salt conditions Compatible with most aqueous buffers
Description Size (mm)  ParticleSize (μm)  Part Number         Agilent Bio SEC-5, 100Å 7.8 x 300 5 5190-2516 Agilent Bio SEC-5, 100Å 7.8 x 150 5 5190-2517 Agilent Bio SEC-5, 100Å 4.6 x 300 5 5190-2518 Agilent Bio SEC-5, 100Å 4.6 x 150 5 5190-2519 Agilent Bio SEC-5, 100Å 7.8 x 50 5 5190-2520   Agilent Bio SEC-5, 150Å 7.8 x 300 5 5190-2521 Agilent Bio SEC-5, 150Å 7.8 x 150 5 5190-2522 Agilent Bio SEC-5, 150Å 4.6 x 300 5 5190-2523 Agilent Bio SEC-5, 150Å 4.6 x 150 5 5190-2524 Agilent Bio SEC-5, 150Å 7.8 x 50 5 5190-2525   Agilent Bio SEC-5, 300Å 7.8 x 300 5 5190-2526 Agilent Bio SEC-5, 300Å 7.8 x 150 5 5190-2527 Agilent Bio SEC-5, 300Å 4.6 x 300 5 5190-2528 Agilent Bio SEC-5, 300Å 4.6 x 150 5 5190-2529 Agilent Bio SEC-5, 300Å 7.8 x 50 5 5190-2530   Agilent Bio SEC-5, 500Å 7.8 x 300 5 5190-2531 Agilent Bio SEC-5, 500Å 7.8 x 150 5 5190-2532 Agilent Bio SEC-5, 500Å 4.6 x 300 5 5190-2533 Agilent Bio SEC-5, 500Å 4.6 x 150 5 5190-2534 Agilent Bio SEC-5, 500Å 7.8 x 50 5 5190-2535   Agilent Bio SEC-5, 1000Å 7.8 x 300 5 5190-2536 Agilent Bio SEC-5, 1000Å 7.8 x 150 5 5190-2537 Agilent Bio SEC-5, 1000Å 4.6 x 300 5 5190-2538 Agilent Bio SEC-5, 1000Å 4.6 x 150 5 5190-2539 Agilent Bio SEC-5, 1000Å 7.8 x 50 5 5190-2540   Agilent Bio SEC-5, 2000Å 7.8 x 300 5 5190-2541 Agilent Bio SEC-5, 2000Å 7.8 x 150 5 5190-2542 Agilent Bio SEC-5, 2000Å 4.6 x 300 5 5190-2543 Agilent Bio SEC-5, 2000Å 4.6 x 150 5 5190-2544 Agilent Bio SEC-5, 2000Å 7.8 x 50 5 5190-2545
Column Specifications     Column Phase Size Exclusion Packing Spherical, high purity, porous silica with a hydrophilic polymeric coating Particle Size 5 μm Pore Structure 100Å, 150Å, 300Å, 500Å, 1000Å, 2000Å Column Exclusion Limits (in Daltons) 100Å MW range: 100 - 100,000 150Å MW range: 500 - 150,000 300Å MW range: 5,000 - 1,250,000 500Å MW range: 15,000 - 5,000,000 1000Å MW range: 50,000 - 7,500,000 2000Å MW range: >10,000,000 pH Stability 2 - 8.5  Operating Temperature Limit Recommended range: 10-30°C, maximum: 80°C Operating Pressure Limit Recommended operating pressure: 137 bar (2,000 psi) Maximum pressure: 240 bar (3,500 psi) Mobile Phase Compatability Recommended: 150 mM phosphate buffer, pH 7.0; other aqueous buffers with high and low salt can be used Working Flow Rate 0.1 - 1.25 mL/min for 7.8 mm ID columns 0.1 - 0.4 mL/min for 4.6 mm ID columns

 

 

 

 

 

 

 

 

 

 

 

 

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