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you’re
in > Products > Agilent Technologies > BioAnalysis > MARS > LC Human 14
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Multiple Affinity Removal LC Column - Human 14
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Simple method removes 14 abundant proteins
In order to more easily isolate and identify proteins in human serum, the Agilent Human 14 Multiple Affinity Removal Column is designed to chromatographically remove fourteen interfering high-abundant proteins from human plasma samples. Removal of these abundant proteins improves the subsequent LC/MS and electrophoretic analysis of the serum sample by effectively expanding the dynamic range of the analysis. |
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- Column eliminates approximately 94% of total protein (albumin, IgG, antitrypsin, IgA, transferrin, haptoglobin, fibrinogen, alpha2-macroglobulin, alpha1-acid glycoprotein, IgM, apolipoprotein Al, apolipoprotein All, complement C3, and transthyretin) when used with the Multiple Affinity Removal System.
- Three column dimensions are available and include 4.6 x 50 mm, 4.6 x 100 mm, and 10 x 100 mm. Custom sizes and spin cartridges are also available.
- Serum injection volume ranges between 20 - 40 µL per run for the 4.6 mm ID, and 160 µL for the 10 mm ID column.
- Optimize sample recovery by utilizing our rapid, single injection protocols and optimized buffers.
- Optimized 2 buffer system allows for highly reproducible re-use of the Columns for over 200 injections – available in a convenient Column Reagent Starter Kit
- Process up to 8,000 µL of plasma/serum over the column lifetime for the 4.6 mm ID columns and 32,000 µL for the 10 mm ID columns.
- Protocols and columns are optimized for use with human physiological samples. Column not recommended for use with mouse serum.
- Column is compatible with most FPLC or HPLC pumps or instruments, including the Agilent 1200/1100 instruments.
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| Description |
Size (mm) |
Part Number |
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| Multiple Affinity Removal Column, Hu-14 |
4.6 x 50 |
5188-6557 |
| Multiple Affinity Removal Column, Hu-14 |
4.6 x 100 |
5188-6558 |
| Multiple Affinity Removal Column, Hu-14 |
10 x 100 |
5188-6559 |
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LC Column Specifications - Human 14
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| Feature |
Specification |
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| Column Dimensions |
4.6 x 50 mm (0.83 mL bed volume) |
| 4.6 x 100 mm (1.66 mL bed volume) |
| 10 x 100 mm (7.8 mL bed volume) |
| Custom sizes available |
| Column body material |
PEEK (polyetheretherketone) |
| End-Fitting material |
PEEK with 2 µm frits |
| Column capacity** |
High-capacity serum load - 4.6 mm ID
- 50 mm column
Hu-14: 20 µL
- 100 mm column
Hu-14: 40 µL
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High-capacity serum load - 10 mm ID
- 100 mm column
Hu-14: 250 µL
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| Injections/Column |
Standard & High-capacity
> 200 |
| Total serum/plasma processed per lifetime |
High-capacity serum load - 4.6 mm ID
- 50 mm column
Hu-14: 4,000 µL
- 100 mm column
Hu-14: 8,000 µL
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High-capacity serum load - 10 mm ID
- 100 mm column
Hu-14: 32,000 µL
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| Max. pressure |
120 bar |
| Operating temperature |
18 - 25oC |
| Column packing material |
Affinity resin |
| Immobilized ligands |
Affinity ligands to human high abundant proteins (albumin, IgG, antitrypsin, IgA, transferrin, haptoglobin, fibrinogen, alpha2-macroglobulin, alpha1-acid glycoprotein, IgM, apolipoprotein AI, apolipoprotein AII, complement C3 and transthyretin) |
| Flow rate range |
0.125 - 3.0 mL/min |
| Shipping solution |
Buffer A with 0.02% sodium azide |
| Shipping temperature |
2 - 8oC - (35 -46oF) |
| Storage temperature |
2 - 8oC - (35 -46oF) |
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**For exact column capacity, consult your column certificate of analysis. Human and mouse sera protein concentrations can vary; injected sample sizes should be adjusted accordingly. Mouse serum capacities were determined using pooled Swiss Webster mouse serum.
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