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you’re
in > Products > Agilent Technologies > BioAnalysis > MARS > LC Human 7
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Multiple Affinity Removal LC Column - Human 7
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Simple method removes 7 abundant proteins
The Multiple Affinity Removal LC Column – Human 7 is designed to chromatographically remove SEVEN interfering high-abundant proteins from human plasma samples. Removal of these abundant proteins improves the subsequent LC/MS and electrophoretic analysis of the serum sample by effectively expanding the dynamic range of the analysis. |
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- Column eliminates approximately 88-92% of total protein (albumin, IgG, IgA, transferrin, haptoglobin, antitrypsin, and fibrinogen) when used with the Multiple Affinity Removal System
- Three column dimensions include 4.6 x 50 mm, 4.6 x 100 mm,
10 x 100 mm. Custom sizes also available.
- Serum injection volume ranges between 30 - 70 µL per run for the 4.6 mm ID, and 250 - 300 µL for the 10 mm ID column
- Optimize sample recovery by utilizing our rapid, single injection protocols and optimized buffers
- Columns designed for over 200 injections
- Process the most plasma/serum over the column lifetime, up to 14,000 µL for the 4.6 mm ID columns and 60,000 µL for the 10 mm ID column.
- Protocols and column are optimized for use with human physiological samples. Column not recommended for use with mouse serum.
- Column is compatible with most LC or HPLC pumps or instruments
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| Description |
Size (mm) |
Part Number |
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| Multiple Affinity Removal Column, Hu-7 |
4.6 x 50 |
5188-6409 |
| Multiple Affinity Removal Column, Hu-7 |
4.6 x 100 |
5188-6410 |
| Multiple Affinity Removal Column, Hu-7 |
10 x 100 |
5188-6411 |
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LC Column Specifications - Human 14
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| Feature |
Specification |
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| Column Dimensions |
4.6 x 50 mm (0.83 mL bed volume) |
| 4.6 x 100 mm (1.66 mL bed volume) |
| 10 x 100 mm (7.8 mL bed volume) |
| Custom sizes available |
| Column body material |
PEEK (polyetheretherketone) |
| End-Fitting material |
PEEK with 2 µm frits |
| Column capacity** |
High-capacity serum load - 4.6 mm ID
- 50 mm column
Hu-7: 30 - 35 µL
- 100 mm column
Hu-7: 60 - 70 µL
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High-capacity serum load - 10 mm ID
- 100 mm column
Hu-7: 250 - 300 µL
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| Injections/Column |
Standard & High-capacity
> 200 |
| Total serum/plasma processed per lifetime |
High-capacity serum load - 4.6 mm ID
- 50 mm column
Hu-7: 7,000 µL
- 100 mm column
Hu-7: 14,000 µL
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High-capacity serum load - 10 mm ID
- 100 mm column
Hu-7: 60,000 µL
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| Max. pressure |
120 bar |
| Operating temperature |
18 - 25oC |
| Column packing material |
Affinity resin |
| Immobilized ligands |
Affinity ligands to human (albumin, IgG, antitrypsin, IgA, transferrin and haptoglobin) or mouse high abundant proteins (albumin, IgG, and transferrin) |
| Flow rate range |
0.25 - 3.0 mL/min |
| Shipping solution |
Buffer A with 0.02% sodium azide |
| Shipping temperature |
2 - 8oC - (35 -46oF) |
| Storage temperature |
2 - 8oC - (35 -46oF) |
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**For exact column capacity, consult your column certificate of analysis. Human and mouse sera protein concentrations can vary; injected sample sizes should be adjusted accordingly. Mouse serum capacities were determined using pooled Swiss Webster mouse serum.
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