Agilent ZORBAX capillary (0.5 mm, 0.3 mm i.d.) and nano (0.1 mm, 0.075 mm i.d.) columns are now available in a wide variety of phases, pore sizes, and dimensions. These columns are ideal for very sample-limited applications because they provide enhanced sensitivity by reducing on-column sample dilution. This high sensitivity can be provided with exceptional reproducibility using Agilent columns and low dispersion HPLC instruments. Reproducibility is a critical issue when working with small sample sizes.