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3100 OFFGEL Fractionator: Improved protein and peptide identification
In the example below, immunodepleted plasma was separated into 24 fractions by OFFGEL electrophoresis (pH3-10) and into 50 fractions by SCX. Subsequent analysis by RP HPLC-Chip/MS revealed that the workflow that included OFFGEL electrophoresis identified twice as many proteins and three times as many peptides. |
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| Part Numbers and Prices |
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| Description |
Part Number |
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Agilent 3100 OFFGEL Fractionator
Includes local controller, preinstalled software and default methods, power cable, two electrode sets, two trays, and optional installation and familiarization service. |
G3100AA |
Kits for OFFGEL electrophoresis mode |
Starter Kit
Fractionation of protein or peptide samples.
Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples: three samples pH 3-10, 12 fractions; three samples pH 3-10, 24 fractions, three samples, pH 4-7, 12 fractions; three samples, pH 4-7, 24 fractions |
5188-6444 |
Low Resolution Kit, pH 3-10
Fractionation of protein or peptide samples into 12 fractions in the pH range 3-10. Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples. |
5188-6425 |
High Resolution Kit, pH 3-10
Fractionation of protein or peptide samples into 24 fractions in the pH range 3-10. Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples. |
5188-6424 |
Low Resolution Kit, pH 4-7
Fractionation of protein or peptide samples into 12 fractions in the pH range 4-7. Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples. |
5188-6427 |
High Resolution Kit, pH 4-7
Fractionation of protein or peptide samples into 24 fractions in the pH range 4-7. Kit includes IPG gel strips, well frames, cover seals, electrode pads, cover fluid and reagents. Kit contains all components for fractionation and in-liquid recovery of 12 samples. |
5188-6426 |
OFFGEL Protein Test Sample
Checkout sample for testing the performance of the Agilent 3100 OFFGEL Fractionator. The checkout sample contains a single protein which will focus into only one fraction when used with the Low Resolution Kit, pH 3-10 (order no. 5188-6425). |
5188-6428 |
Spare parts |
Tray Set
Four replacement trays. |
G3100-60007 |
Electrode Assembly
One tray, one fixed and one movable electrode. |
G3100-60002 |
12-Well Frame Set
Set includes replacement parts for fractionation of 12 samples: well frames (12 wells), cover seals, electrode pads, glycerol solution, cover fluid and manual. Does not include IPG gel strips, ampholytes or reagents. To be used with 13-cm IPG gel strips and respective ampholytes. For use with 5188-6444, 5188-6425, 5188-6427. |
5188-8012 |
24-Well Frame Set
Set includes replacement parts for fractionation of 12 samples: well frames (24 wells), cover seals, electrode pads, glycerol solution, cover fluid and manual. Does not include IPG gel strips, ampholytes or reagents. To be used with 24-cm IPG gel strips and respective ampholytes. For use with 5188-6444, 5188-6424, 5188-6426. |
5188-8013 |
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