Poroshell 300SB-C18, C8, C3 and 300Extend - Applications

Applications Some key application areas for Poroshell columns are those with very large molecules, including polypeptides and proteins (up to 1000 kDA), monoclonal antibodies and intact proteins by LC and LC/MS.

Application notes

Fast Separation of Large and Heterogeneous Proteins Using ZORBAX Poroshell C18, C8, and C3 Phases Choosing a ZORBAX Poroshell Phase (C3, C8, or C18) for Fast Separation of Monoclonal Antibodies Rapid HPLC Analysis of Monoclonal Antibody IgG1 Heavy Chains Using ZORBAX Poroshell 300SB-C8 Rapid HPLC Analysis of Monoclonal Antibody IgG1 Light Chains Using ZORBAX Poroshell 300SB-C8 Effect of Flow Rate on Ultra-Fast Separations of Proteins on Porous and Superficially Porous Particle Columns Using Poroshell 300SB-C18 for High-Sensitivity, High-Throughput Protein Analysis on the Agilent LC/MSD Decreasing Analysis Time Using Poroshell 300SB-C18 in Analysis of a Protein Digest Effect of Temperature on Ultrafast Separation of Macromolecules

Bonded Phase Pore Size Temp. Limits pH range Endcapped           SB-C18, C8, C3 300Å 90°C 1.0 - 8.0 No 300Extend 300Å 40°C above pH 8 60°C below pH 8 2.0 - 11.0 Yes

Poroshell (Particle Size 5µm) Ordering Information

Features Advantages Benefits Unique, 300Å porous shell on a 5µm solid particle Fast kinetics Fast, high resolution of protein and other macromolecule separations Higher surface area than non-porous particles for higher capacity Lower backpressure Fast separations without associated higher pressures Uses StableBond chemistry   Long column life at low pH; acidic mobile phases (e.g. TFA) Extremely stable at low pH Excellent reproducibility for low pH protein separations, column-to-column, batch-to-batch Extremely stable at high temperatures Faster run times using elevated temperatures Uses C18, C8 and C3 bonding Additional selectivity with different temperatures Available in 300Extend-C18 Additional selectivity option, can be used at high pH Uses high purity Zorbax Rx-Sil (99.995% pure) sols Less acidic (fewer metals) Good peak shape 5 µm particle size High resolution High strength, high-pressure packing Better resolution

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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