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Agilent Bio-Monolith HPLC Columns
Bio-Monolith HPLC Columns Applications |
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| Column |
Description |
Key Applications |
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| Bio-Monolith QA |
The quaternary amine bonded phase (Strong Anion Exchange) is fully charged over a working pH range of 2-13, binding bio-molecules with negative charges. The Bio-Monolith QA column is designed for a wide range of applications, particularly process monitoring and quality control of viruses, DNA and large proteins. |
- HSA Purity
- Adenovirus process monitoring and quality control
- Monitoring DNA impurity removal
- Monitoring endotoxin removal IgM purification monitoring and quality control
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| Bio-Monolith DEAE |
The diethylaminoethyl bonded phase (Weak Anion Exchange) offers increased selectivity of bio-molecules with negative charge over a working pH range of 3-9. Applications include process monitoring and quality control of bacteriophage and plasmid DNA purifications. |
- Process monitoring and quality control of bacteriophage purification
- Process monitoring and quality control of plasmid DNA purification
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| Bio-Monolith SO3 |
The sulfonyl bonded phase (Strong Cation Exchange) is fully charged over a working pH range of 2-13, binding bio-molecules with positive charges. Applications include fast and high resolution analytical separations of large bio-molecules such as proteins and antibodies. |
- Fast and high resolution analytical separations of large molecules such as proteins, antibodies
- Hemoglobin A1c fast analytics
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Bio-Monolith
Protein A |
The Protein A affinity column is designed for the analytical separation of all IgG (human and mouse), except for IgG class3. Enables rapid separation and quantitation of IgG. |
- Quantitative Determination of Human IgG (fermentation titer calculation)
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| *Store columns at 4-8 degrees Celsius before and after initial use |
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| Specification |
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| Property |
Specification |
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| Dimensions |
5.2 mm x 4.95 mm |
| Column volume |
100 µL |
| Maximum pressure |
150 bar (15 MPa, 2200 psi) |
| Temperature min/max |
Working: 4°C - 40°C
Storage: 4°C - 30°C |
| Recommended pH |
Working range: 2 - 13
Cleaning-in-place: 1 - 14 |
| Materials of construction |
Hardware: Stainless steel
Packing: poly (glycidyl methacrylate-co-ethylene dimethacrylate) highly porous monolith
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| Color ring identifier |
Bio-Monolith SO3: Red
Bio-Monolith QA: Blue
Bio-Monolith DEAE: Green
Bio-Monolith Protein A: White |
| Shelf life/expiration date |
Protein A: 12 months
SO3, QA, DEAE: 24-36 months |
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