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you’re
in > Products > Agilent Technologies > BioAnalysis > MARS > mRP-C18 High-Recovery Protein Column
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mRP-C18 High-Recovery Protein Column
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Separate complex mixtures
The mRP-C18 High-Recovery Protein Column is an LC column designed for sample desalting, followed by chromatographic separation or fractionation of the complex protein samples (like immunodepleted serum or plasma proteins) with extremely high recovery of sample when compared to conventional RP HPLC columns.
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- Greater than 95-99% protein sample recovery have been observed with immunodepleted serum using the Agilent Multiple Affinity Removal System - LC Column.
- Can load up to 380 µg of total protein mass without reducing chromatographic resolution of the proteins.
- Four column dimensions include 0.5 x 100 mm, 2.1 x 75 mm, 4.6 x 50 mm, and 9.4 x 50 mm with PEEK (polyetheretherketone) end-fittings and 2 µm PEEK-encapsulated titanium frits.
- Column packed with macroporous C18-bonded ultrapure 5 µm particle silica designed to reduce or eliminate strong adsorption of proteins.
- Maximum operating pressure of 250 bar (4000 psi).
- Compatible with water and all common organic solvents.
- Simple mobile phases and gradient and use of standard HPLC equipment make protein fractionation or desalting easy and reproducible.
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| Description |
Size (mm) |
Part Number |
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mRP-C18 High-Recovery Protein Column
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0.5 x 100 |
5188-6510 |
mRP-C18 High-Recovery Protein Column
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2.1 x 75 |
5188-6511 |
mRP-C18 High-Recovery Protein Column
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4.6 x 50 |
5188-5231 |
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| Column Specifications |
| Feature |
Specification |
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| Column Dimensions |
0.5 x 100 mm, 2.1 x 75 mm, 4.6 x 50 mm |
| Operating pH range |
0.9 - 8.0 (although pHs of > 6.0 are not recommended) |
| Column packing material |
Column body and end-fittings: PEEK (polyetheretheketone). End-fittings have standard 1/16" tubing 10-32 threads as per standard HPLC columns and Multiple Affinity Removal Columns
Frits: PEEK-encapsulated titanium frits (2 µm)
Packing:macroporous C18-bonded ultrapure 5 µm particle silica designed to reduce or eliminate strong adsorption of proteins. |
| Shipping solvent |
A new column contains a mixture of methanol and water. Initially, care should be taken not to pass any mobile phase through the column that might cause a precipitate |
| Solvent Compatibility |
Compatible with water and all common organic solvents, including N, N-dimethyl-formamide and dimethysulfoxide |
| Max. Pressure |
Do not exceed 250 bar (4000 psi) |
| Column packing material |
Affinity resin |
| Operating Temperatures |
Maximum operating temperature at pH < 5 is 80°C, for pH 5 - 8 it is 40°C |
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