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Grace HPLC Column Selection Guide

Grace® Key Column Families Single-Source Solution for Discovery to Recovery Applications

HPLC is commonly used in a wide range of applications, including drug discovery and purification for the pharmaceutical and biotechnology industries, environmental analysis, forensics, petrochemical analysis, food, cosmetics, and vitamins. The combined premier product lines of Alltech®, Grace®, Jones Chromatography™, and Vydac® create a single-source solution for HPLC columns and accessories from discovery to recovery.

 
To find appropriate HPLC column phases for compounds of interest, start by selecting the
molecule size, then find the appropriate phases based on the application.
 

Molecule Size


Large Molecules (MW > 2,000)


Small Molecules (MW < 2,000)

Large Molecule Separations (MW > 2,000)

Applications Recommended Columns
   
Simple Enzymatic Digests (< 12 proteins) Vydac® 218 MS
Complex Enzymatic Digests (≥ 12 proteins) Vydac® Everest® C18
Biomolecules (0-5K MW) Vydac® 218 MS, Vydac® 238 MS
Biomolecules (5-10K MW) Vydac® 208 MS
Biomolecules (>10K MW) Vydac® 214 MS, ProZap™ C18
Undenatured Intact Proteins Vydac® 214 MS, ProZap™ C18
Antibodies Vydac® 214 MS, ProZap™ C18
Oligonucleotides Vydac® 214 MS, ProZap™ C18
Oligosaccharides

Vydac® Everest® C18, ProZap™ C18


Small Molecule Separations (MW < 2,000)

  • Pharmaceuticals
  • Food/Flavor/Fragrance
  • Environmental
  • Biotech/Life Sciences
  • Medical/Clinical
  • Biofuels

Pharmaceuticals Applications


  • VisionHT™ UHPLC Columns
  • Platinum™ HPLC Columns
  • GraceSmart™ HPLC Columns

Food/Flavor/Fragrance Applications

Applications Recommended Columns
   
Carbohydrates Prevail™ Carbohydrate ES
Organic Acids Prevail™ Organic Acid , Alltima™ HP C18 AQ
Amino Acids Prevail™ C18, GraceSmart™ C18, Alltima™ HP HILIC
Lipids/Oils Alltima™ HP C18 High Load
Nutraceuticals GraceSmart™ C18 HPLC
Vitamins - Water Soluble Prevail™ C18, GraceSmart™ C18, Alltima™ HP C18 AQ
Vitamins - Fat Soluble Alltima™ HP C18 High Load, Prevail™ C18

Environmental Applications

Applications Recommended Columns
   
Pesticides GraceSmart™ C18
Herbicides GraceSmart™ C18
PAHs Vydac® 201TP, 202TP

Small Molecule Biotech/Life Science Applications

Applications Recommended Columns
   
Amino Acids Prevail™ C18, GraceSmart™ C18, Alltima™ HP HILIC
Nucleotides, Nucleogides Platinum™ EPS C18, GraceSmart™ C18
Lipids, Oils Alltima™ HP C18 Hi-Load

Medical/Clinical Applications

Applications Recommended Columns
   
Amino Acids Prevail™ C18, GraceSmart™ C18, Alltima™ HP HILIC
Vitamins - Water Soluble Prevail™ C18, GraceSmart™ C18, Alltima™ HP C18 AQ
Vitamins - Fat Soluble Alltima™ HP C18 High Load, Prevail™ C18

Biofuels Applications

Applications Recommended Columns
   
Mono-, di-, tri-acylglycerides Alltima™ HP C18 High Load
FAMEs Alltima™ HP C18 High Load
Free Glycerol Alltima™ HP C18 High Load
Sterol Glucosides Alltima™ HP C18 High Load
Phospholipids Alltima™ HP Silica, Prevail™ Silica
Carbohydrates Prevail™ Carbohydrate ES


 



 
 
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