High-speed, high-efficiency columns for fast protein analysis
Short 10 and 20mm ProZap™ columns with 1.5μm particles provide high-speed protein and life science separations. Sharp, efficient peaks maximize method sensitivity.
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Optimization of Gradient Parameters for Fast Protein Analysis
Short ProZap™ columns are the perfect tool for fast reversed-phase protein separations. Under gradient conditions, longer columns only increase run time and do not increase resolution to improve the separation. Proteins adsorb at the head of the column and then desorb once the critical mobile phase concentration is reached. Since the proteins do not interact with the full length of the packed bed, short columns are sufficient for full resolution. Therefore, proteins of broad molecular weight ranges can be separated in less than one minute by combining short columns, higher flow rates, and fast, modified gradients. For best results high-pressure mixing should be used with fast gradients.
Differentiated Phases: C18
Specifications: Sub 2μm spherical silica, proprietary
bonding and endcapping, 500Å pore size
Format: Expedite™ |
