This one-day course introduces the fundamentally important concepts associated with troubleshooting HPLC analysis including hardware basics, separation issues, peaks shape and baseline issues and more.
All components of the HPLC system and associated problems are systematically studied. The use of chromatograms as diagnostic tools is explored in depth, including baselines, peak shape and retention time inconsistencies, spurious peaks, etc.
Suitable as a refresher for the more experienced analytical chemist or as an invaluable introduction to the technique for those with limited experience, this course provides an invaluable insight into HPLC principles and practice.
Who is this course for
This course is designed for those relatively new to HPLC, and ideally will follow-on from attendance on the fundamental HPLC training course.
No previous knowledge of chromatography is necessary, but basic experience of chemistry is beneficial.
What you will learn
- How the chromatographic process takes place
- The architecture of a modern HPLC system
- Working principle and problems associated with each module of the HPLC system
- How to use chromatograms to troubleshoot chromatographic problems
- Best practices for column use and care
- Best practice in system maintenance and performance evaluation