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Introduction to Bio-Molecule Mass Spectrometry class room course
1 Day Course

Crawford Scientific are delighted to offer a new course directly aimed at those working on bio-molecules analysis and in particular within the field of proteomics. A more advanced course covering Protein Analysis and Post-Translational Modifications etc. will be available. This course can also be customised to meet you exact requirements. Contact us at to discuss your training needs.

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Introduction to Bio-Molecule Mass Spectrometry

9:00 – 9:15 am   Assemble and tea/coffee
9:15 – 9:40 am   Introductions and course overview
9:40 – 10:40 am    Session 1 – Biological mass spectrometry theory and hardware
Electrospray, APCI and MALDI
Traps, quads and Tof analysers
Ion fragmentation methods
MS scanning modes
Instrument figures of merit
10:40 – 11:00 am    Refreshment break
11:00 – 12:00 pm   Session 2 – Sample preparation techniques
Gel-based separations
Complex mixtures
Reduction/alkylation and enzymatic digestion
SPE sample clean-up (C18 and HILIC)
12:00 – 1:00 pm   Lunch
1:00 – 2:00 pm     Session 3 – Nano LC
Components of nanoLC (UPLC) systems  
Trapping configurations and on-column loading
Gradient conditions for peptide separation
2:00 – 2:20 pm     Refreshment break
2:20 – 3:20 pm   Session 4 – Database searching
Principles of protein identification
Types of search engine     
Using Mascot
Peak list generation
Statistical Scoring
False Discovery Rates
3:20 – 4:00 pm   Course summary, course quiz, certificates and feedback forms

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