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APPLICATION NOTES
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| Analgesic Tablet - USP |
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Column: PrincetonSPHER C18 100A 5u
Catalog No: 100046-03501
Dimensions: 100 x 4.6 mm
Eluent: 69/28/3 A/B/C
A: Water
B: Methanol
C: Acetic Acid
Flow Rate: 2.0 mL/min
Temperature: 45 degrees C
Detection: UV at 254 nm
Injection: 5 uL (1 mg/mL sample)
1. Acetaminophen
2. Caffeine
3. Aspirin
4. Benzoic Acid |
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| Antiarrthyhmic Compounds |
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Column: PrincetonSPHER C18 100A 5u
Catalog No: 150046-03501
Dimensions: 150 x 4.6 mm
Eluent: 60/40 A/B
A: 20mM NH2H2PO4 - pH 3.2
B: Acetonitrile
Flow Rate: 1.5 mL/min
Temperature: 40 degrees C
Detection: UV at 280 nm
Injection: 10 uL (1 mg / mL sample)
1. Phenytoin
2. Propanolol
3. Quinidine
4. Dilatizem
5. Verapamil
6. Nicardipine |
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| Antidepressants |
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Column: PrincetonSPHER
Ultima-C18-WP
Catalog No: 150046-01524
Dimensions: 150 x 4.6 mm
Eluent: 40/60 A/B
A: 25mM KH2PO4 - pH 6.6
B: Acetonitrile
Flow Rate: 2.0 mL/min
Temperature: 40 degrees C
Detection: UV at 230 nm
Injection: 10uL (1 mg/mL sample)
1. Protriptyline
2. Desipramine
3. Nortriptyline
4. Doxepin
5. Imipramine
6. Amitriptyline
7. Trimipramine
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| Aromatic Acids |
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Column: PrincetonSPHER C18 100A 5u
Catalog No: 100046-03501
Dimensions: 100 x 4.6 mm
Eluent: 50/50 A/B
A: 0.1M H3PO4 - pH 2.0
B: Methanol
Flow Rate: 1.5 mL/min
Temperature: Ambient
Detection: UV at 240 nm
Injection: 5 uL (1 mg/mL sample)
1. p-Aminobezoic Acid
2. p-Hydroxybenzoic Acid
3. o-Aminobenzoic Acid
4. Benzyl Alcohol
5. Salicylic Acid |
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| Beta Blockers |
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Column:PrincetonSPHER 2-Ethylpyridine
Catalog No: 150046-01577
Dimensions: 150 x 4.6 mm
Eluent: 80/20 A/B
A: 20mM K2HPO4 - pH 6.6
B: Acetonitrile
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detection: UV at 273 nm
Injection: 10uL (1 mg/mL sample)
1. Metoprolol
2. Propanolol
3. Pindolol |
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| Bovine Serum Albumin |
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Column: PrincetonSPHER SEC/Aq
300A 10u
Catalog No: 300078-08072
Dimensions: 300 x 7.8 mm
Eluent: 9.5 g/L Dulbecco Buffer-pH 7.3
Flow Rate: 1.0 mL/min
Temperature: 20 degrees C
Detection: UV at 210 nm
Injection: 30 uL |
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| Buckminsterfullerenes |
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Column: PrincetonSPHER C18 60A 5u
Catalog No: 250046-01501
Dimensions: 250 x 4.6 mm
Eluent: 60/40 A/B
A: Toluene
B: Acetonitrile
Flow Rate: 1.0 mL/min
Temperature: 20 degrees C
Detection: UV at 320 nm
Injection: 5 uL (1 mg/mL sample)
1. C60
2. C70
3. C78
4. C84 |
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| Creatine and Creatinine |
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Column: PharmaBOND C18 125A 10u
Catalog No: 300039-05001
Dimensions: 300 x 3.9 mm
Eluent: 70/30 A/B*
A: 0.1% H3PO4
B: Acetonitrile *with 1.4 mg/mL
Dodecyl Sulfate Sodium
Flow Rate: 1.0 mL/min
Temperature: 20 degrees C
Detection: UV at 210 nm
Injection: 5 uL (1 mg/mL sample)
1. Creatine
2. Creatinine |
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| "Combinatorial" Test Mixture |
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Column: PrincetonSPHER OmniSEP
Catalog No: 050046-01570
Dimensions: 50 x 4.6 mm
Eluent: Gradient*
A: 0.1% TFA
B: Acetonitrile
* 0-4 min 100% A - 0% A, 0% B -
100% B linear; 4-5 min 100% B;
5-6 min 0% A - 100% A, 100% B -
0% B.
Flow Rate: 2.5 mL/min
Temperature: 20 degrees C
Detection: UV at 220 nm
Injection: 5 uL (1 mg mL sample)
1. Sodium Nitrate
2. 1-Hydroxy-7-aza-benzotriazole
3. 4-Methoxybenzene sulfonamide
4. Methyl 3-amino-
2-thiophenecarboxylate
5. 4-Aminobenzophenone
6. Benzo[a]pyrene
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| Diuretics |
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Column: PrincetonSPHER C18 100A 5u
Catalog No: 150046-03501
Dimensions: 150 x 4.6 mm
Eluent: 60/40 A/B
A: 20mM NH2H2PO4 - pH 3.2
B: Acetonitrile
Flow Rate: 1.5 mL/min
Temperature: 40 degrees C
Detection: UV at 280 nm
Injection: 10 uL (1 mg/mL sample)
1. Hydrochlorothiazide
2. Chlorthalidone
3. Diazoxide
4. Furosemide
5. Bendroflumethazide
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| Explosives |
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Column: PrincetonSPHER C18 60A 5u
Catalog No: 250046-01501
Dimensions: 250 x 4.6 mm
Eluent: 70/30 A/B
A: Acetonitrile
B: Water
Flow Rate: 1.5 mL/min
Temperature: 30 degrees C
Detection: UV at 254 nm
Injection: 10 uL (1 mg/mL sample)
1. 2,4-DNT
2. 4,4’-DPA
3. 4N-DPA
4. NNO-DPA
5. 2,4-DPA
6. 2-2’-DPA
7. 2,4-DPA
8. DPA
9. 2N-DPA
10.Dibutyl Phthalate |
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| Flavonoids |
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Column: PrincetonSPHER FO* 60A 5u
Catalog No: 250046-01542
Dimensions: 250 x 4.6 mm
Eluent: 60/40 A/B
A: 0.1% H3PO4
B: Acetonitrile
Flow Rate: 1.0 mL/min
Temperature: 20 degrees C
Detection: UV at 254 nm
Injection: 10uL (1 mg/mL sample)
1. Quercetin
2. Naringenin
*FO = Fluorooctyl
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| Organic Acids |
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Column: PrincetonSPHER OmniSEP
Catalog No: 250046-01590
Dimensions: 250 x 4.6 mm
Eluent: 0.1% H3PO4
Flow Rate: 1.0 mL/min
Temperature: 30 degrees C
Detection: UV at 210 nm
Injection: 20 uL (1 mg/mL sample)
1. Tartaric Acid
2. Formic Acid
3. Malic Acid
4. Malonic Acid
5. Lactic Acid
6. Maleic Acid |
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| Chromatography of a Polyanionic Polymer (Avgmw~700K) |
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Columns: PrincetonSPHER SEC/Aq 60Å, 120Å, 300Å, 500Å,
1000Å, 4000Å
Dimensions: 300 x 7.8mm (all columns)
Eluent: 9.5 g/L Dulbecco Buffer - pH 7.0
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detection: UV @ 254 nm |
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| Proteins |
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Column: PrincetonPREP C18 150A 18u
Catalog No: 250046-06801
Dimensions: 250 x 4.6 mm
Eluent: Gradient*
A: 0.1% TFA
B: Acetonitrile 0.1% TFA * 0-5 min
75% A / 25% B; 5-25 min 75%
A - 25% A, 25% B - 75% B linear;
25-30 min hold.
Flow Rate: 1.5 mL/min
Temperature: 20 degrees C
Detection: UV at 210 nm
Injection: 5 uL (1 mg/mL sample)
1. Ribonuclease
2. Insulin
3. Lysozyme
4. Myoglobin
5. Myoglobin Impurity |
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| SEC of Aromatic Acids |
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Column: PrincetonSPHER SEC/Aq
60Å 10µ
Catalog No: 300078-01072
Dimensions: 300 x 7.8 mm
Eluent: 9.5 g/L Dulbecco Buffer, pH 7.0
Flow Rate: 1.0 mL/min
Temperature: Ambient
Detection: UV at 254 nm
Injection: 10 uL (1 mg/mL sample)
1. p-Hydroxybenzoic Acid
2. p-Toluenesulfonic Acid |
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| SEC of Proteins |
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Column: PrincetonSPHER SEC/Aq
300A 10u
Catalog No: 300078-08072
Dimensions: 300 x 7.8 mm
Eluent: 0.015 M NaH2PO4, 0.015 M
Na2HPO4, 0.15 M NaCl, pH 6.8
Flow Rate: 1.0 mL/min
Temperature: 20 degrees C
Detection: UV at 220 nm
Injection: 25 uL
1. Thyroglobulin (MW 670,000)
2. Ovalbumin (MW 44,000)
3. Cyanocobalamin (MW 1,350) |
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| Steroids |
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Column: PrincetonSPHER C18 100A 5u
Catalog No: 250046-03501
Dimensions: 250 x 4.6 mm
Eluent: 66/34 A/B
A: Acetonitrile
B: Water
Flow Rate: 1.0 mL/min
Temperature: 20 degrees C
Detection: UV at 200 nm
Injection: 10uL (1 mg/mL sample)
1. Ethinyl Estradiol
2. Norethindrone |
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| Taxol |
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Column: PrincetonSPHER PFP* 60A 5u
Catalog No: 250046-01506
Dimensions: 250 x 4.6 mm
Eluent: 50/50 A/B
A: Acetonitrile
B: Water
Flow Rate: 1.0 mL/min
Temperature: 30 degrees C
Detection: UV at 230 nm
Injection: 10 uL (1 mg/mL sample)
1. Taxol
*PFP = Pentafluorophenyl |
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| Uracil and 5-Fluorouracil |
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Column: PrincetonSPHER DIOL
60A 5u
Catalog No: 250046-01509
Dimensions: 250 x 4.6 mm
Eluent: 80/20 A/B
A: Acetonitrile&
B: Water
Flow Rate: 1.0 mL/min
Temperature: 20 degrees C
Detection: UV at 254 nm
Injection: 10uL (1 mg/mL sample)
1. 5-Fluorouracil
2. Uracil |
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| USP XXIII - Acetaminophen |
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Column: PharmaBOND C18 125A 10u
Catalog No: 300039-05001
Dimensions: 300 x 3.9 mm
Eluent: 60/36/4; Methanol, water,
Tetrahydrofuran w/ 0.1% H3PO4
and 4.4mg/mL Docusate Sodium
Flow Rate: 1.5 mL/min
Temperature: Ambient
Detection: UV at 280 nm
Injection: 15uL (1 mg/mL sample)
Sample: 0.85 g Acetaminophen/Codeine
tablet (#3) in 250 mL mobile phase
1. Acetaminophen
2. Codeine |
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| Vanillins |
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Column: PrincetonSPHER FO* 60A 5u
Catalog No: 150046-01542
Dimensions: 150 x 4.6 mm
Eluent: 30/70 A/B
A: Acetonitrile
B: 0.1% H3PO4
Flow Rate: 1.0 mL/min
Temperature: 20degrees C
Detection: UV at 254 nm
Injection: 5 uL (1 mg/mL sample)
1. Vanillic Acid
2. Vanillin
3. Ethyl Vanillin
*FO = Fluorooctyl |
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| Water Soluble Vitamins |
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Column: PrincetonSPHER C18 100A 5u
Catalog No: 150046-03501
Dimensions: 150 x 4.6 mm
Eluent: 0-5 min 100% A; 5-20 min
100% - 0% A / 0% -100% B Linear;
20-25 min 100% B
A: 20mM K2HPO4 - pH 6.6
B: 80/20 K2HPO4 (pH 6.6) / ACN
Flow Rate: 1.0 mL/min
Temperature: 30 degrees C
Detection: UV at 254 nm
Injection: 10uL (1 mg/mL sample)
1. L-Ascorbic Acid
2. Nicotinic Acid
3. Pyroxidine
4. Nicotinamide
5. Folic Acid
6. Cyanocobalamin
7. Riboflavin
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