|
|
|
|
| you’re
in home > Products > Manufacturers > Shodex > KW-800 KW-400 |
| |
Shodex KW-800 and KW400 HPLC columns |
 |
|
Protein Analysis by GFC
KW-800 and KW400 series HPLC columns are specifically designed for protein analysis by aqueous size exclusion chromatography (GFC). They are packed with porous silica particles coated with a hydrophilic copolymer.
|
|
| |
|
|
| KW-800 |
KW400 |
| |
|
- High protein recovery rates
- Highest sample load
- Recommended for older HPLC systems
|
- High protein recovery rates
- 3 to 4 times greater sensitivity
- 1.5 times higher TPN
- Faster analyses
- ~60% less eluent consumption
|
|
| Shodex KW-800 |
The packing material of the Shodex PROTEIN KW-800 series is based on silica particles whose surface is coated with a hydrophilic polymer. This column is for aqueous SEC, and indexed in the Shodex catalogue under Gel filtration chromatography (GFC). It enjoys a positive reputation thanks to its high recovery ratio of proteins and peptide. The PROTEIN KW-800 series comprises three kinds of columns, differing in pore and particle size. |
| |
KW-800 Series
Size (mm) |
Plate Number
(TP/column) |
Exclusion Limit |
Particle
Size (μm) |
Pore Size (Å) |
Part Number |
| Pullulan |
Protein |
Max. |
Avg. |
| |
|
|
|
|
|
| 8.0 x 300 |
> 21,000 |
60,000 |
150,000 |
5 |
400 |
150 |
KW-802.5 |
| 8.0 x 300 |
> 21,000 |
170,000 |
700,000 |
5 |
1,000 |
300 |
KW-803 |
| 8.0 x 300 |
> 16,000 |
500,000 |
1,000,000 |
7 |
1,500 |
500 |
KW-804 |
| 6.0 x 50 |
(Guard column) |
- |
- |
7 |
- |
- |
KW-G |
|
| |
| Shodex KW400 |
The Shodex KW400-4F series is the high-performance semi-micro column version of the KW-800 series. The theoretical plate number and the sensitivity are improved by reducing the diameter of the packed particle to 3 micrometers. KW405-4F, the newest addition to the KW400 line-up, has a larger pore size than any columns in the existing KW-800 series, and can analyze proteins whose molecular weight is larger than 1,000,000. It enables the scientist to carry out the analysis of protein complexes. |
| |
KW-400 Series
Size (mm) |
Plate Number
(TP/column) |
Exclusion Limit |
Particle
Size (μm) |
Pore Size (Å) |
Part Number |
| Pullulan |
Protein |
Max. |
Avg. |
| |
|
|
|
|
|
| 4.6 x 300 |
> 35,000 |
60,000 |
150,000 |
3 |
400 |
150 |
KW-402.5-4F |
| 4.6 x 300 |
> 35,000 |
150,000 |
600,000 |
3 |
800 |
250 |
KW-403-4F |
| 4.6 x 300 |
> 25,000 |
500,000 |
1,000,000 |
5 |
1,500 |
500 |
KW-404-4F |
| 4.6 x 300 |
> 25,000 |
1,300,000 |
20,000,000 |
5 |
2,000 |
1,000 |
KW-405-4F |
| 4.6 x 10 |
(Guard column) |
- |
- |
5 |
- |
- |
KW-G |
|
| |
| |
| |
|
|
|
