Sielc - Obelisc - LiSC - Living Separation Cell

Similarities to a living cell

The cell internal environment is very different from the external environment (mobile phase);
The pore walls form the dimensions and structure of the liquid separation cell;
The cell internal environment has stationary (bound) and exchangeable (mobile) components;
The pH and ionic strength inside the cell differs from outside the cell;
The pore opening acts as a membrane channel for the analytes;
The cell responds to changes in external environment with physical and chemical changes
These changes include conformational changes in the long hydrophobic and hydrophilic chains, degree of ionization, charge distribution and counter-ion properties.

Three main characteristics of the cell make it different from traditional chromatography materials

Significantly higher ionic strength in the cell vs. mobile phase ion strength, providing quick mass transfer of charged analytes in and out of the cell even with low concentration of buffer in the mobile phase.
The stationary phase occupies the entire volume of the cell, not just the walls as in other stationary phases, providing high capacity of the phase.
Positive and negative charges of the cell ligand separated by a long organic chain allow both positive and negative charges to simultaneously participate in electrostatic interaction.

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