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Sielc - Primesep - Pi-Pi Interaction

HPLC separation of aromatic compounds on Primesep P columns.
 

SIELC Technologies has developed a new class of stationary phases, which provides three interactions with analytes:

  • reverse phase
  • pi-pi
  • strong cation exchange

Depending on analyte properties, one, two, or all three interactions can be applied to the separation.

 


Schematic of Primesep P stationary phase chemistry

 

This stationary phase facilitates the separation of structural isomers of aromatic compounds.

Additional pi-pi interaction creates a difference in the bind state for structural isomers and often resolves critical pairs of compounds. The degree of pi-pi interaction can be adjusted by varying the amount of acetonitrile in the mobile phase.

When methanol is used as an organic modifier, the highest degree of aromatic interaction can be achieved.

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