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you’re in > Products > Manufacturers > Sielc > Promix |
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Sielc - Promix |
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Promix is a new type of chromatography stationary phase designed for separation of proteins and peptides. There are three types of phases that SIELC specialists developed for separation of this type of bio-molecule. Promix SP is a column for very short and/or very hydrophilic peptides. Typically it is difficult to retain such peptides on RP column. The Promix SP column offers an improved retention profile and alternative selectivity if compared with RP separation. Promix AP is a column designed for short basic peptides. This phase allows the retention of peptides with several histidine and lysine residues. |
| A pH gradient is the most convenient way to control retention of basic peptides on this column. Use a buffer with a neutral or slightly acidic pH (4.5 - 7) in the beginning of the gradient, and use a more acidic buffer (pH 2 - 3.5) in the end of the gradient elution. Promix MP is a column designed for medium size hydrophobic peptides. Typical applications include protein digest which increases peak capacity of the digest by 30 to 50%. This column offers alternative selectivity if compared with simple RP columns. Typically difficult to separate pairs of peptides can be resolved on this stationary phase. |
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Features & Benefits
- 2-D HPLC with a Single Column
- Ionic Interactions
- Reversed-Phase
- Separate peptides and proteins that differ only by a single amino acid
- Enhanced selectivity for closely related peptides and proteins.
- Increased peak capacity compared to reversed-phase and ion exchange.
- Increased LC-MS sensitivity using formic acid and ammonium acetate.
- Completely Scalable from capillary to prep
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| For ordering information and pricing for Promix HPLC Column please click here |
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Promix SP is a stationary phase for small peptides under 1 kDa with PI value below 6.0.
- Reverse phase and ion-exchange mechanism of interaction.
- Use acidic mobile phase pH from 2 to 5.5.
- Use ion strength at least 20 mM.
- 100Å pore size is available.
Promix SP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um Pores: 100 Å |
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Promix AP is a stationary phase for small and medium size peptides under 3 kDa with PI value below 7.0.
- Reverse phase and ion-exchange mechanism of interaction.
- Use acidic mobile phase pH from 2 to 5.5.
- Use ion strength at least 20 mM.
- 100Å and 300Å pore sizes are available.
Promix AP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 300 Å , 100 Å |
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Promix MP is a stationary phase for medium size proteins and peptides from 1 to 10 kDa.
- Broad pI value.
- Reverse phase and ion-exclusion mechanism of interaction.
- Use acidic mobile phase pH from 2 to 5.5.
- Use ion strength at least 1 mM.
- 300Å and 800Å pore sizes are available.
Promix MP columns are available in all standard dimensions
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 800 Å , 300 Å |
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Promix LP is no longer available but Promix MP is available at 800Å pore size |
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| For ordering information and pricing for Promix HPLC Column please click here |
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