TFA Removal
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SiliaBond Carbonate (R66030B) |
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Standard Quantities Available:
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- 25 g |
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- 100 g |
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- 500 g |
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- 1 kg |
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Trifluoroacetic acid is certainly the most commonly used ion-pairing agent for the separation of peptides in reversed-phase ion-pairing chromatography.
This popularity is explained by the fact that:
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it is volatile and easily removed; |
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it has low absorption within detection wavelengths; |
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it has a proven history;
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| The role of TFA is to act as buffer, keeping the charge on the analyte and avoiding precipitation, to impart some hydrophobicity to the amino groups, and to neutralize cationic charges. |
| After HPLC separation, the peptide is isolated by evaporation of the solvent in its TFA salt form. Libraries of peptides stored this way exhibit reduced stability. It is hence necessary to use a method to free-base the peptides prior to their storage. SiliaBond Carbonate from SiliCycle is an efficient and convenient solution to this problem. It has successfully been used to free-base a library of TFA salt of amines (Org. Lett. 4 (2002), 1167-1170) |