BioBasic SEC Capillaries Columns

BioBasic SEC columns are available in four pore sizes (60, 120, 300 and 1000Å) to cater for separations of samples from 100 to 10,000,000 molecular weight. The columns are based on porous silica particles coated with with a hydrophillic polymer for superior column lifetimes. They give superior resolution with 100% aqueous or organic eluents, and do not swell or shrink with changes in solvent, showing higher peak capacityies than polymer-based SEC columns. The polymer coating eliminates secondary interactions so that samples are separated based stricly on their size in solution. BioBasic SEC columns are ideal for high efficiency gel filtration separation of proteins and other biological molecules where the absence of secondary interactions such as adsorption, is essential for accurate analysis. The columns are offered in a standard 300 x 7.8mm size, and also 150 x 7.8mm for faster separations, as well as a 30mm length guard column to maximize column lifetimes.

Improved reproducibility, efficiency and column lifetimes Range of stationary phases to optimize selectivity Powerful resolution, even on short column lengths

Phase Particle size (µm) Pore size (Å) Column size Inner diameter x length Product Number           SEC 5 60 150x7.8mm 73305-157846 120 150x7.8mm 73405-157846 300 150x7.8mm 73505-157846 1000 150x7.8mm 73605-157846 60 300x7.8mm 73305-307846 120 300x7.8mm 73405-307846 300 300x7.8mm 73505-307846 1000 300x7.8mm 73605-307846 60 30x7.8mm 73305-037821 120 30x7.8mm 73405-037821 300 30x7.8mm 73505-037821 1000 30x7.8mm 73605-037821

Column Pore Size (Å) Molecular Weight (kDa) Pore Volume Proteins Pullalans Polyethylene Oxides/Glycols BioBasic SEC 60 60 0.1 - 6 0.70cc/gram 0.3 - 6 0.1 - 4 BioBasic SEC 120 120 0.1 - 50 1.00cc/gram 0.3 - 12 0.4 - 10 BioBasic SEC 300 300 1 - 500 0.90cc/gram 1 - 100 2 - 100 BioBasic SEC 1000 1000 20 - 4000 0.90cc/gram 20 - >1000 not recommended

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