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you’re in > Products > Manufacturers > Tosoh > TSK-GEL Anion Exchange COLUMNS
 

TSK-GEL Anion Exchange Columns
  • TSKgel Q- and DNA-STAT columns provide high efficiency separations at short analysis time.
  • TSKgel DNA-NPR columns are ideal for PCR fragment analysis.
  • TSKgel SuperQ-5PW columns have higher capacity than TSKgel DEAE-5PW due to novel bonding chemistry, effective pore size is smaller for SuperQ-5PW.
  • Pore structure and bonding chemistry of TSKgel BioAssist Q columns provide high capacity for small to very large MW proteins and nucleic acids.
  • BioAssist columns are packed in 4.6 mm ID or 10 mm ID PEEK hardware. Other columns are available in glass and stainless steel for analytical, semi-preparative and preparative
    applications.
  • Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is roughly double that of the DEAE-5PW due to the smaller pore size and larger surface area.
  • TSKgel DEAE-5PW and DEAE-2SW columns are available in 2 mm ID format for mass spec applications.
  • Specialty columns for analysis of mono- and disaccharides and sugar alcohols are also available.
 
Please click here to see TSK-GEL Anion Exchange Applications.
 
 

Specialty Columns

Analyses of monosaccharides, disaccharides, and sugar alcohols can be performed on PS-DVB columns, either by isocratic (TSKgel Sugar AXI) or by gradient (TSKgel Sugar AXG) analysis.
Saccharides are retained on Sugar AX columns following the formation of negatively charged complexes with boric acid at alkaline pH. Figure 7 shows the separation of twelve mono- and
di-saccharides. The strong anion exchange TSKgel SAX column can be used for the separation of isomerized sugars, alcohols, and low molecular weight organic acids.
 
Description
ID (mm)
Length (cm)
Particule Size (μm)
Number Theoretical Plates

Flow Rate (mL/min)

Max Pressure Drop (kg/cm2)
Part #
Range Max
Glass columns
DEAE-5PW 1000 Å 5.0 5.0 10 ≥ 700 0.5-0.8 1.0 15
13061
DEAE-5PW 1000 Å 8.0 7.5 10 ≥ 1,300 0.5-1.0 1.2 10
08802
DEAE-5PW 1000 Å 20.0 15.0 13 ≥ 3,000 4.0-6.0 8.0 15
14016
SuperQ-5PW 1000 Å 8.0 7.5 10 ≥ 1,300 0.5-1.0 1.2 20
13386
 
PEEK Columns
BioAssist Q, 4000 Å 4.6 5.0 10 ≥ 500 0.3-1.0 1.2 25
19685
BioAssist Q, 4000 Å 10 10.0 13 ≥ 500 1.0-5.0 7.0 25
21410
 
Stainless steel columns: polymer-based
Q-STAT, nonporous 3.0 3.5 10 ≥ 200     100
21960
Q-STAT, nonporous 4.6 10.0 7 ≥ 2,000     100
21961
Q-STAT, nonporous 4.6 10.0 5 ≥ 4,000     150
21962
                 
DEAE-NPR, nonporous 4.6 3.5 2.5 ≥ 1,300 1.0-1.5 1.6 200
13075
DNA-NPR, nonporous 4.6 7.5 2.5 ≥ 6,000 0.5-1.0 1.5 300
18249
                 
QC-PAK GFC 200 7.8 15 5 ≥ 10,000 0.5-1.0 1.2 40
16215
QC-PAK GFC 300 7.8 15 5 ≥ 10,000 0.5-1.0 1.2 40
16049
                 
DEAE-5PW, 1000 Å 2.0 7.5 10 ≥ 1,300 0.05-0.10 0.12 15
18757
DEAE-5PW, 1000 Å 7.5 7.5 10 ≥ 1,300 0.5-1.0 1.2 15
07164
DEAE-5PW, 1000 Å 21.5 15.0 13 ≥ 3,000 4.0-6.0 8.0 25
07574
DEAE-5PW, 1000 Å 55.0 20.0 20 ≥ 1,500 20.0-40.0 50.0 4
07930
SuperQ-5PW, 1000 Å 7.5 7.5 10 ≥ 1,300 0.5-1.0 1.2 20
18257
SuperQ-5PW, 1000 Å 21.5 15.0 13 ≥ 3,000 4.0-6.0 8.0 20
18387
                 
Sugar AXI,
60 Å		 4.6 15.0 8 ≥ 3,700 0.2-0.4 0.5 30
08639
Sugar AXI,
60 Å		 4.6 15.0 10 ≥ 2,700 0.2-0.4 0.5 20
08640
                 
SAX 6.0 15.0 5 ≥ 2,000 0.5 - 1.0 1.2 150
07157
                 
Stainless steel columns: silica-based
DEAE-2SW, 125 Å 2.0 25.0 5 ≥ 5,000 0.12-0.17 0.22 130
18761
DEAE-2SW, 125 Å 4.6 25.0 5 ≥ 5,000 0.6-0.8 1.0 150
07168
DEAE-3SW, 250 Å 7.5 7.5 10 ≥ 1,300 0.5-1.0 1.2 20
07163
                 
Guard column products
DEAE-NPR 4.6 0.5 5 Guard column for P/N 13075
17088
DNA-NPR 4.6 0.5 5 Guard column for P/N 18249
18253
           
SuperQ-5PW     20 Guardgel Kit for P/N 18257
18388
SuperQ-5PW     20 Guardgel Kit Glass for P/N 18386
18389
SuperQ-5PW     20 Guardgel Kit for P/N 18387
18390
           
DEAE-5PW     20 Guardgel Kit for P/N 18257
07210
DEAE-5PW 2.0 1.0 10 Guard cartridge for P/N 18757
42152
           
DEAE-5PW     20 Guardgel Kit Glass for P/N 13061 and 08802
08806
DEAE-5PW 20 2.0 13 Guard column Glass for P/N 14016
14466
           
DEAE-5PW     20 Guardgel Kit Prep for P/N 07574
16092
DEAE-5PW 45 5.0 20 Guard column for P/N 07930
07928
           
DEAE-SW     20 Guardgel Kit for P/N 07168 and 07163
07648
DEAE-2SW 2.0 1.0 5 Guard cartridge for P/N 18761
42154
           
Guard cartridge holder(2.0mm ID, 1.5cm L) For all 2mm ID guard cartridges
19308
 
 
Applications
 

Nonporous TSKgel STAT Anion Exchange Columns

STAT columns are available in various column formats and particle sizes to perfectly match specific application needs. For fast and ultra-fast analysis anion and cation exchange columns in 3 mm ID and 3.5 cm length are packed with 10 µm particles.
They are ideally suited for rapid candidate screening or process monitoring. 4.6 mm ID and 10 cm length columns packed with 7 µm particles are designed for high resolution IEC separation for example for the separation of nucleic acids, mAb variants, PEGylated protein or protein aggregates.
Figure 1 compares the high resolution separation of nucleotides on a 10 cm length column to the high throughput separation on a 3.5 cm length column (analysis performed on prototype columns). Polymer-based Anion Exchange Columns.
Figure 1
   

BioAssist Q TSKgel

BioAssist Q is suitable for use in systems that are designed for laboratory or semi-preparative applications. Figure 2 demonstrates the performance enhancement of TSKgel BioAssist Q over a competitive product when operated side-byside on an FPLC system. TABLE I shows typical dynamic binding capacities on BioAssist Q relative to competitive products.
 
Table 1
Figure 2
   

SuperQ-5PW and DEAE-5PW

Figure 3 shows the analysis of a 16-mer morpholine oligonucleotide on TSKgel SuperQ-5PW column using a NaCl gradient in a 10 mmol/L sodium hydroxide mobile phase. Figure 4 shows the fractionation of high molecular weight E. coli RNA on TSKgel DEAE-5PW, effectively utilising the large 1000 Å pores of this base resin.
Figure 3
Figure 4
   

DEAE-NPR and DNA-NPR

Because of their small (2.5 µm) particle size, non porous resin (NPR) columns excel in rapid separations of large biomolecules such as DNA digests.
A chromatogram of a standard Hae III digest of pBR322 DNA on TSKgel DEAE-NPR, protected by a guard column, is shown in Figure 5.
To achieve better resolution for PCR fragment analysis we recommend the use of TSK-GEL DNA-NPR columns, which are 7.5 cm long and 4.6 mm wide, providing higher efficiency in a longer column.
 
Figure 5
   
   

Silica-based Anion Exchange Columns

TSK-GEL 2SW-type columns provide high performance separations of small ionic solutes. The increased solubility of the silica backbone above pH 7 limits the use of the TSK-GEL 2SWtype columns to acidic or neutral mobile phases. This restricts method development and requires special cleaning procedures when compared to the more robust TSK-GEL 5PW-type polymerbased columns.
High performance analyses of small anionic species are best performed on small pore silica-based anion exchangers, such as TSKgel DEAE-2SW. This is demonstrated in Figure 6. The 250 Å pore size TSKgel DEAE-3SW column is used for separating peptides, low MW proteins and DNA fragments.

Specialty Columns

Analyses of monosaccharides, disaccharides, and sugar alcohols can be performed on PS-DVB columns, either by isocratic (TSKgel Sugar AXI) or by gradient (TSKgel Sugar AXG) analysis. Saccharides are retained on Sugar AX columns following the formation of negatively charged complexes with boric acid at alkaline pH.
Figure 7 shows the separation of twelve mono- and di-saccharides. The strong anion exchange TSKgel SAX column can be used for the separation of isomerized sugars, alcohols, and low molecular weight organic acids.
 
Figure 6
Figure 7
   
 
 
 
  

 

 

 

 

 

 

 

 

 

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