TSK-GEL Cation Exchange Columns

TSKgel SP-STAT and CM-STAT nonporous columns provide high efficiency separation at short analysis time. Pore structure and bonding chemistry of TSKgel BioAssist S provides high capacity for medium to large MW proteins. BioAssist columns are packed in 4.6 mm ID or 10 mm ID PEEK hardware. Other columns are available in glass and stainless steel for analytical, semi-preparative and preparative applications. Binding capacity for small to medium size proteins on TSKgel CM-3SW is approximately double that of TSKgel CM-5PW due to the smaller pore size and larger surface area. The TSKgel SP-5PW column is available in 2 mm ID format for LC-MS applications.

Please click here to see TSK-GEL Cation Exchange Applications.

Description ID (mm) Length (cm) Particule Size (μm) Number Theoretical Plates Flow Rate (mL/min) Max Pressure Drop (kg/cm2) Part # Range Max Glass columns CM-5PW 1000 Å 5.0 5.0 10 ≥ 700 0.5-0.8 1.0 15 14010 CM-5PW 1000 Å 8.0 7.5 10 ≥ 1,300 0.5-1.0 1.2 10 14011 CM-5PW 1000 Å 20.0 15.0 13 ≥ 2,500 4.0-6.0 8.0 15 14012 CM-5PW 1000 Å 5.0 5.0 10 ≥ 700 0.5-0.8 1.0 15 13062 CM-5PW 1000 Å 8.0 7.5 10 ≥ 1,300 0.5-1.0 1.2 10 08803 CM-5PW 1000 Å 20.0 15.0 13 ≥ 3,000 4.0-6.0 8.0 15 14017   PEEK Columns BioAssist S, 1300 Å 4.6 5.0 7 ≥ 1,500 0.3-0.8 1.0 25 19686 BioAssist S, 1300 Å 10 10.0 13 ≥ 3,000 1.0-5.0 7.0 25 21411   Stainless steel columns: polymer-based CM-STAT, nonporous 3.0 3.5 10 ≥ 200     100 21965 CM-STAT, nonporous 4.6 10.0 7 ≥ 2,000     100 21966 SP-STAT, nonporous 3.0 3.5 10 ≥ 200     100 21963 SP-STAT, nonporous 4.6 10.0 7 ≥ 2,000     100 21964                   CM-5PW, 1000 Å 7.5 7.5 10 ≥ 1,300 0.5-1.0 1.2 15 13068 CM-5PW, 1000 Å 21.5 15.0 13 ≥ 2,500 4.0-6.0 8.0 25 14021                   SP-5PW, 1000 Å 2.0 7.5 10 ≥ 1,300 0.05-0.10 0.12 10 18758 SP-5PW, 1000 Å 7.5 7.5 10 ≥ 1,300 0.5-1.0 1.2 15 07161 SP-5PW, 1000 Å 21.5 15.0 13 ≥ 3,000 4.0-6.0 8.0 25 07575 SP-5PW, 1000 Å 55.0 20.0 20 ≥ 1,500 20.0-40.0 50.0 4 07934                   SP-NPR, nonporous 4.6 3.5 2.5 ≥ 1,300 1.0-1.5 1.6 200 13076                   SCX (Na+) 6.0 15.0 5 ≥ 2,000 0.5 - 1.0 1.2 150 07156 SCX (H+) 7.8 30.0 5 ≥ 12,000 0.5 - 1.0 1.2 50 07158                   Stainless steel columns: silica-based SP-2SW, 125 Å 4.6 25.0 5 ≥ 5,000 0.6-0.8 1.0 150 07165 CM-2SW, 125 Å 4.6 25.0 5 ≥ 5,000 0.6-0.8 1.0 150 07167 CM-3SW, 250 Å 7.5 7.5 10 ≥ 1,300 0.5-1.0 1.2 20 07162                   Guard column products CM-5PW     10 Guardgel Kit for P/N 13068 13069 CM-5PW     20 Prep Guardgel Kit for P/N 14021 16094             CM-5PW     20 Guardgel Kit Glass for P/N 14010 and 14011 14024 CM-5PW 20.0 2.0 13 Prep Guardgel Kit Glass for P/N 14012 14468             SP-5PW     20 Guardgel Kit for P/N 07161 07211 SP-5PW 2.0 1.0 10 Guard cartridge for P/N 18758 42153 SP-5PW     20 Guardgel Kit Glass for P/N 13062 and 08803 08807 SP-5PW 20 2.0 13 Guard column Glass for P/N 14017 14467             SP-5PW     20 Guardgel Kit Prep for P/N 07575 16093 SP-5PW 45 5.0 20 Guard column for P/N 07934 07932             DEAE-SW     20 Guardgel Kit for P/N 07168 and 07163 07648 DEAE-2SW 2.0 1.0 5 Guard cartridge for P/N 18761 42154             CM-SW     20 Guardgel Kit for P/Ns 07167 and 07162 07650             Guard cartridge holder(2.0mm ID, 1.5cm L) For all 2mm ID guard cartridges 19308

Applications       TSKgel BioAssist S TSKgel BioAssist S is suitable for use in systems that are designed for HPLC, laboratory, or semi-preparative applications. The large pore size of the TSKgel BioAssist S resin provides high dynamic capacity due to novel bonded phase design (see Table 1). Figure 1 demonstrates these features for the analysis of bromelain, a proteolytic enzyme that is used as a nutritional supplement. Bromelain is a basic glycoprotein with a MW of 33 kDa and a pI of 9.55.   Table1 Figure 1     TSKgel SP-5PW and TSKgel CM-5PW Differences in selectivity between strong (TSKgel SP-5PW) and weak (TSKgel CM-5PW) cation exchangers are demonstrated in Figure 2, which is a separation of globular proteins. The purification of 200mg of crude lipoxidase on a 21.5 mm ID TSKgel SP-5PW column is illustrated in Figure 3. Scale-up is simplified as only the particle size changes from 10 μm (7.5 mm ID) to 13 μm (21.5 mm ID) or 20 μm (55 mm ID) columns. Figure 2 Figure 3     SuperQ-5PW and DEAE-5PW Figure 4 shows the analysis of a 16-mer morpholine oligonucleotide on TSKgel SuperQ-5PW column using a NaCl gradient in a 10 mmol/L sodium hydroxide mobile phase. Figure 5 shows the fractionation of high molecular weight E. coli RNA on TSKgel DEAE-5PW, effectively utilising the large 1000 Å pores of this base resin. Figure 4 Figure 5

 

 

 

 

 

 

 

 

 

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