Fast Reversed Phase Columns TSK-GEL ODS-140HTP |
TSK-GEL ODS-140HTP columns were developed for use
in high throughput applications, including drug discovery,
pharmacokinetics and peptide digest separations. They are
available in 2.1 mm ID columns with 5 cm and 10 cm lengths.
TSKgel ODS-140HTP columns are packed with 2.3 μm particles,
providing high resolution and short analysis times at moderate
pressure. The lower pressure drop reduces the burden on the
hardware, allowing TSKgel ODS-140 HTP columns to be used with either UPLC (up to 9000 psi) or conventional HPLC systems.
The backpressure of a TSKgel ODS-140 HTP column is less than
half of the pressure of a sub-2 μm column of the same dimensions. (cf figure below) |
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- Moderate pressure at high flow rates
- High resolution and high efficiency
- High throughput applications
- Compatible with HPLC and UPLC systems
- Moderate carbon content
- Polylayer bonding chemistry
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| Two applications have been selected and can be found below |
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| Description |
ID (mm) |
Length (cm) |
Particule Size (μm) |
Pore Size (Å) |
Matrix |
Part # |
| Stainless steel columns |
| TSKgel ODS-140HTP |
2.1 |
5.0 |
2.3 |
140 |
Silica |
21927
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| TSKgel ODS-140HTP |
2.1 |
10.0 |
2.3 |
140 |
Silica |
21928
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| Backpressure of a TSKgel ODS-140 HTP compared to sub-2 µm columns |
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Column: TSKgel ODS-140HTP, 2.3 μm, (2.1mm ID x 5 cm, 10 cm)
Sub-2 μm ODS columns (2.1mm ID x 5 cm)
Eluent: H2O/ACN (50/50) + 0.1% TFA
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| Applications |
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Excellent resolution at high speed can be achieved on a TSKgel
ODS-140HTP column wih the separation of a ß-lactoglobulin
trytic digest (see Figure below). Peak capacity improved when using
a longer gradient time. |
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| Separation of a ß-lactoglobulin trytic digest using TSKgel
ODS-140HTP |
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Column: TSKgel ODS-140HTP, 2.3 μm, (2.1mm ID x 5 cm)
Eluent: A: H2O/ACN (95/5) + 0.1% TFA
B: H2O/ACN (50/50) + 0.1% TFA
Flow rate: 1.0 mL/min
Detection: UV@220nm
Temperature: 40ºC
Injection volume: 10 μL
Gradient: 0-100%B (Linear gradient)
Gradient time: A: 10 min, B: 2.5 min
Sample: β-lactoglobulin tryptic digest
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In Vietnamese and Chinese traditional medicine, hot aqueous extract of Crinum latifolium is used because of its antitumor activity. Crinum latifolium is thought to possess antiviral and immunostimulative properties and shows immunomodulatory properties in human peripheral blood mononuclear cells.
The analysis of products derived from plant extracts is a challenging chromatographic task.
Due to the high number ob components the column needs to provide a high peak capacity, as shown in figure below. |
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Column: TSKgel ODS-140HTP 2.3 μm, 2.1 mm ID x 10 cm L
Sample: Crinum latifolium L extract, 2 μl
Eluent: A: water B: acetonitrile
Gradient: 0 min (5%B) 1.2 min (5%B) 4 min (30%B)
15 min (68%B) 15.1 min (100%B) 20min (100%B)
Flow rate: 0.4 ml/min
Temp.: 40°C
Detection: UV @ 220 nm |
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