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TSK-GEL Super-ODS TSK-GEL Super-Octyl TSK-GEL Super Phenyl Columns

The silica particles used in Super series columns are monodisperse spherical 2 μm beads with 110 Å pores. The highlights are:

  • TSKgel Super-ODS, Super-Octyl and Super-Phenyl packings are bonded with, respectively, C18, C8 and phenyl functional groups. The bonded phases have a polymeric structure. An exhaustive endcapping reaction minimizes the presence of residual silanol groups
  • 2 μm particles provide superior resolution and speed, as well as improved sensitivity
  • Pressure drop is not excessive due to the monodisperse particle size distribution
  • Stainless steel columns are available with 4.6 mm and 2 mm ID formats
 
 
Applications
 
  • Recommended for small molecular weight compounds (<10,000Da) such as peptides, amino acids, tryptic digests, nucleotides, pharmaceutical molecules, and food and beverage samples.
 
Optimising Results with Super Series Columns
 
Super series columns can be used on a regular HPLC system if the dead volume is minimized, although optimal results are obtained with an HPLC system designed for 2 mm or smaller ID
columns (click here for more information).

Description
ID (mm)
Length (cm)
Particule Size (μm)
Number Theoretical Plates

Flow Rate (mL/min)

Max Pressure Drop (kg/cm2)
Part #
Range Max
Stainless steel columns
Super-ODS, 110 Å
1.0
5.0
2
≥ 15,000
0.03-0.05
0.06
150
20015
Super-ODS, 110 Å
1.0
10.0
2
0.15-0.18
0.06
150
20016
Super-ODS, 110 Å
2.0
5.0
2
≥ 6,000
0.15-0.20
0.25
250
19541
Super-ODS, 110 Å
2.0
10.0
2
≥ 12,000
0.15-0.20
0.25
250
19542
Super-ODS, 110 Å
4.6
5.0
2
≥ 8,000
1.0-2.5
4.0
300
18154
Super-ODS, 110 Å
4.6
10.0
2
≥ 16,000
1.0-2.5
4.0
300
18197
                 
Super-Octyl, 110 Å
2.0
5.0
2
≥ 5,000
0.15-0.20
0.25
150
20013
Super-Octyl, 110 Å
2.0
10.0
2
≥ 15,000
0.15-0.20
0.25
300
20014
Super-Octyl, 110 Å
4.6
5.0
2
≥ 8,000
1.0-2.5
4.0
300
18275
Super-Octyl, 110 Å
4.6
10.0
2
≥ 16,000
1.0-2.5
4.0
300
18276
                 
Super-Phenyl, 110 Å
2.0
5.0
2
≥ 3,000
0.15-0.20
0.25
80
20017
Super-Phenyl, 110 Å
2.0
10.0
2
≥ 6,000
0.15-0.20
0.25
150
20018
Super-Phenyl, 110 Å
4.6
5.0
2
≥ 8,000
1.0-2.5
4.0
300
18277
Super-Phenyl, 110 Å
4.6
10.0
2
≥ 16,000
1.0-2.5
4.0
300
18278
Guard column products
ODS-120T
2.0
1.0
2
Guard Cartridge (pk/3) for 2 mm ID Super
19672
ODS-120T
4.0
4.0
2
Guard Cartridge (pk/3) for all 4.6 mm ID (Super-ODS, -Octyl, -Phenyl)
18207
Cartridge holder For 2.0 mm ID Guard cartridges
19308
Cartridge holder For 4.0 mm ID Guard cartridges
18206
 

Recommendations

The following recommendations are for 4.6 mm ID columns. Use proportionately lower values for 2 mm ID columns.
1. A guard filter is highly recommended to reduce particulate contamination from the sample or system components.
2. Keep sample volume less than 10 μL.
3. To ensure minimal extra-column volume, keep tubing as short as possible (extra-column volume less than 5 μL between column and detector).
4. Conventional 0.1 mm ID connecting tubing may be used (0.005 ).
5. The smallest detector time constant should be selected (if possible, less than 50 ms).
6. The detector flow cell should be 2 μL or less for best results. A standard HPLC flow cell (10 μL) can be used as an alternative, however, it is recommended that the heating coil is removed.

 
 
 
  

 

 

 

 

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