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| you’re in > Products > Manufacturers > Tosoh > TSK-GEL SW COLUMNS |
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TSK-GEL SW, SWxl and SuperSW Gel Filtration columns |
TSK-GEL SW-type columns (SW, SWXL and SuperSW column lines) are all based on spherical silica particles with very high internal pore volumes.
For properties and separation ranges for TSK-GEL SW-type packings please click here
The highlights are:
- Silica particles in SW-type columns are chemically bonded with a stationary phase containing polar diol groups.
- SW-type columns feature low residual adsorption and very high pore volumes, which are essential characteristics of high performance gel filtration columns.
- SW and SWXL columns lines are available in three pore size ranges with nominal pore sizes of 125 Å, 250 Å and 450 Å. SuperSW and QC-PAK column lines are available in 125 Å and 250 Å pores.
- SW columns are packed with 10 micron (G2000SW and G3000SW) or 13 micron (G4000SW) particles. SWXL columns contain 5 micron (G2000SWXL and G3000SWXL) or 8 micron (G4000SWXL) particles. SuperSW columns contain 4 micron particles (SuperSW2000 and SuperSW3000)
- All SW-type columns are available in stainless steel hardware.
SW and QC-PAK columns are also available in glass, while SWXL columns are also available in PEEK hardware.
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| For Applications and recommendations for TSK-GEL SW-type Gel Filtration columns please click here |
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| For calibration curves for TSK-GEL SW-type Gel Filtration columns please click here |
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Description |
ID (mm) |
Length (cm) |
Particule Size (μm) |
Number Theoretical Plates |
Flow Rate (mL/min)
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Max Pressure Drop (kg/cm2)
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Part # |
| Range |
Max |
| Glass columns |
| QC-PAK GFC 200GL |
8.0 |
15 |
5 |
≥ 10,000 |
0.5-1.0 |
1.2 |
40 |
16214 |
| QC-PAK GFC 300GL |
8.0 |
15 |
5 |
≥ 10,000 |
0.5-1.0 |
1.2 |
40 |
16216 |
| G2000SW |
8.0 |
30 |
10 |
≥ 10,000 |
0.4-0.8 |
0.8 |
20 |
08799 |
| G3000SW |
8.0 |
30 |
10 |
≥ 10,000 |
0.4-0.8 |
0.8 |
20 |
08800 |
| G3000SW |
8.0 |
30 |
13 |
≥ 8,000 |
0.4-0.8 |
0.8 |
20 |
08801 |
| G4000SW |
20.0 |
30 |
13 |
≥ 6,000 |
3.0-6.0 |
8.0 |
8 |
14464 |
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| Stainless steel columns |
| SuperSW2000 |
4.6 |
30 |
4 |
≥ 30,000 |
0.1-0.35 |
0.4 |
30 |
18674 |
| SuperSW3000 |
1.0 |
30 |
4 |
≥ 18,000 |
0.016 |
0.02 |
60 |
21845 |
| SuperSW3000 |
2.0 |
30 |
4 |
≥ 25,000 |
0.065 |
0.075 |
90 |
21485 |
| SuperSW3000 |
4.6 |
30 |
4 |
≥ 30,000 |
0.1-0.35 |
0.4 |
120 |
18675 |
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| G2000SWXL |
7.8 |
30 |
5 |
≥ 20,000 |
0.5-1.0 |
1.2 |
70 |
08540 |
| G3000SWXL |
7.8 |
30 |
5 |
≥ 20,000 |
0.5-1.0 |
1.2 |
70 |
08541 |
| G4000SWXL |
7.8 |
30 |
5 |
≥ 16,000 |
0.5-1.0 |
1.2 |
35 |
08542 |
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| QC-PAK GFC 200 |
7.8 |
15 |
5 |
≥ 10,000 |
0.5-1.0 |
1.2 |
40 |
16215 |
| QC-PAK GFC 300 |
7.8 |
15 |
5 |
≥ 10,000 |
0.5-1.0 |
1.2 |
40 |
16049 |
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| G2000SW |
7.5 |
30 |
10 |
≥ 10,000 |
0.5-1.0 |
8.0 |
10 |
05788 |
| G3000SW |
7.5 |
30 |
10 |
≥ 10,000 |
0.5-1.0 |
8.0 |
15 |
05789 |
| G4000SW |
7.5 |
30 |
13 |
≥ 8,000 |
0.5-1.0 |
8.0 |
10 |
05790 |
| G2000SW |
7.5 |
60 |
10 |
≥ 20,000 |
0.5-1.0 |
8.0 |
20 |
05102 |
| G3000SW |
7.5 |
60 |
10 |
≥ 20,000 |
0.5-1.0 |
8.0 |
30 |
05103 |
| G4000SW |
7.5 |
60 |
13 |
≥ 16,000 |
0.5-1.0 |
8.0 |
20 |
05104 |
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| G2000SW |
21.5 |
30 |
10 |
≥ 10,000 |
3.0-6.0 |
8.0 |
10 |
06727 |
| G3000SW |
21.5 |
30 |
10 |
≥ 10,000 |
3.0-6.0 |
8.0 |
15 |
06728 |
| G4000SW |
21.5 |
30 |
13 |
≥ 8,000 |
3.0-6.0 |
8.0 |
10 |
06729 |
| G2000SW |
21.5 |
60 |
13 |
≥ 20,000 |
3.0-6.0 |
8.0 |
20 |
05146 |
| G3000SW |
21.5 |
60 |
13 |
≥ 20,000 |
3.0-6.0 |
8.0 |
30 |
05147 |
| G4000SW |
21.5 |
60 |
17 |
≥ 16,000 |
3.0-6.0 |
8.0 |
20 |
05148 |
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| G2000SW |
55.0 |
30 |
20 |
≥ 400 |
25.0-40.0 |
50.0 |
10 |
07428 |
| G3000SW |
55.0 |
30 |
20 |
≥ 4,000 |
15.0-25.0 |
50.0 |
10 |
07481 |
| G2000SW |
55.0 |
60 |
20 |
≥ 750 |
20.0-30.0 |
35.0 |
15 |
07429 |
| G3000SW |
55.0 |
60 |
20 |
≥ 9,000 |
15.0-25.0 |
50.0 |
15 |
07482 |
| PEEK Columns |
| BioAssist G2SWXL |
7.5 |
30 |
5 |
≥ 20,000 |
0.5-1.0 |
8.0 |
70 |
20027 |
| BioAssist G3SWXL |
7.5 |
30 |
5 |
≥ 20,000 |
0.5-1.0 |
8.0 |
70 |
20026 |
| BioAssist G4SWXL |
7.5 |
30 |
8 |
≥ 16,000 |
0.5-1.0 |
8.0 |
35 |
20025 |
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| Guard column products |
| SW |
8.0 |
4.0 |
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Glass Guard column, for 8mm ID Glass columns |
08805 |
| SW |
20.0 |
4.0 |
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Glass Guard column, for 8mm ID Glass columns |
14465 |
| SuperSW |
4.6 |
3.5 |
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Guard column for SuperSW 4.6mm ID columns (contains SuperSW3000 packing) |
18762 |
| SWXL |
6.0 |
4.0 |
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Guard column for all SWXL columns (contains 3000SWXL packing) |
08543 |
| SWXL |
3.2 |
4.0 |
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Guard column for all BioAssist SWXL, PEEK columns |
18008 |
| SW |
7.5 |
7.5 |
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For all 7.5 mm ID SW columns |
05371 |
| SW |
21.5 |
7.5 |
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For all 21.5 mm ID SW columns |
05758 |
| SW |
45.0 |
5.0 |
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For 55 mm ID SW columns |
07427 |
| Bulk packing |
| SWXL |
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5 |
Top-Off, 1g wet gel for SWXL and QC-PAK |
08544 |
| SW |
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10 |
Top-Off, 1g wet gel for all 7.5 mm ID SW |
06819 |
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Recommendations for TSK-GEL SW series selection
Samples of unknown molecular weight
TSKgel G3000SWXL is the ideal scouting column. If the protein of
interest elutes near the exclusion volume, then G4000SWXL is the
logical next step. Conversely, if the protein of interest elutes near
the end of the chromatogram, try the G2000SWXL.
Proteins (general)
Choose one of the TSK-GEL SWXL columns using the calibration
curves on page 34 to select the appropriate pore size based on
knowledge or estimate of protein size.
Monoclonal antibodies
TSKgel G3000SWXL is commonly used for quality control. TSKgel
SuperSW3000 is utilized when sample is limited or at very low
concentration.
Peptides
TSKgel G2000SWXL is the first selection for the analysis of
peptides. TSKgel SuperSW2000 is utilized when sample is limited
or at very low concentration.
Other
The use of TSK-GEL SuperSW columns requires optimization of
the HPLC system with respect to extra-column band broadening.
Capillary tubing ID, injection volume, detector cell volume, and
detector time constant all need to be reduced to fully benefit
from the high column efficiency and small peak volumes of the
SuperSW columns. Use SW columns when not sample limited or
when larger amounts of sample need to be isolated.
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| Applications of TSK-GEL SW-type Gel Filtration columns |
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Proteins
The effect of different concentrations of surfactant on the
separation of membrane proteins is seen in Figure 1. As the
concentration of octaethyleneglycol dodecylether increases to
0.05%, the main peak becomes sharper and recovery increases.
The TSKgel SuperSW3000 provides an excellent high resolution
separation of IgG1 from mouse ascites fluid as can be seen in
Figure 2. |
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Figure 1 |
Figure 2 |
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| Enzymes |
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Mobile phase conditions in GFC are optimized to ensure little or no interaction of the sample with the packing material. This gentle technique allows for high recovery of enzymatic activity. For example, crude samples of peroxidase and glutathione S-transferase were separated in only 15 minutes on a TSKgel G3000SWXL column and activity recovery was 98% and 89%, respectively. The elution profiles of the separations in Figure 3 show that all of the activity eluted in a narrow band of about 1.5 mL.
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Figure 3 |
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| For calibration curves for TSK-GEL SW-type Gel Filtration columns please click here |
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