| Technology
YMC, well known for its experience in DAC column technology, both in terms of hardware and packing procedures, has modified its robust DAC-column packing knowledge for semi-preparative column applications. In combination with a new hardware design, YMC-Actus columns achieve an outstanding durability and efficiency.
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Durability
Rapid pressure changes under high-speed gradient conditions may lead to column degradation and loss of column performance.
With YMC-Actus, a new hardware and packing technology has been applied to these semi-preparative columns to provide a uniform packing density which results in a longer lifetime than conventional semi-preparative columns.
In order to determine the quality of the packing, the column performance was evaluated every 100 runs with a sequential high throughput gradient.
YMC-Actus columns offer outstanding efficiency without compromising resolution.
Furthermore, YMC-Actus columns provide reliable results, even after exposure to severe, rapid gradient conditions and multiple injections. |
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| Sequential gradient test |
Column: 50 x 20 mm i.d., 5 μm
or 50 x 19 mm i.d., 5 μm
Eluent: A) water B) methanol
Gradient: 5% B (0-0.5 min), 5-95% B (0.5-3.1 min),
95% B (3.1-3.6 min), 5% B (3.6-4.0 min)
Flow rate: 50 ml/min
Temperature: ambient |
| Column performance test |
Column: 50 x 20 mm i.d., 5 μm
or 50 x 19 mm i.d., 5 μm
Eluent: methanol / water (70/30)
Flow rate: 10 ml/min
Sample: Naphthalene |
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| Excellent durability and efficiency under fast gradient condition at high flow rate |
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| Glycyrrhizic acid in herb medicine |
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Eluent:
A) water/acetic acid (99/1)
B) methanol/acetic acid (99/1)
20%B (0-2min), 20-45%B (2-7min), 45%B (7-10min)
Temperature:
ambient
Detection:
UV at 250 nm
Sample:
water/methanol/acetic acid extract of commercially available herb medicine (0.1g/ml) |
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| R&D Kits To minimise the problems that the process of scale-up may introduce, YMC offers YMC R&D kits, which
consist of one analytical column and one YMC-Actus column packed with exactly the same packing material
from the same production lot. Therefore no further method development is needed, just a simple linear
scale-up calculation.
Outstanding separation of highly polar compounds |
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| Crude synthetic 30mer oligonucleotide |
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Eluent:
A) 10mM DBA-acetic acid (pH6.0)/methanol (60/40)
B) 10mM DBA-acetic acid (pH6.0)/methanol (20/80)
10-35%B (0-20min)
Temperature:
ambient
Detection:
UV at 269 nm
Sample:
synthetic oligonucleotide (100µm) |
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| Excellent separations of compounds with similar structure |
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| Capsaicinoids in red pepper |
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Eluent:
A) methanol/water/TFA (50/50/0.1)
B) methanol/TFA (100/0.1)
0-30%B (0-5min), 30%B (5-10min)
Temperature:
25°C for 50 x 4.6 mm i.d.
ambient for 50 x 20 mm i.d.
Detection:
UV at 280 nm
Sample:
methanol extract of a commercial red pepper (1g/3ml)
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