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ZORBAX Rapid Resolution High Definition (RRHD) Columns - Analyse more samples, faster

 

 Achieve true high-definition    separations without sacrificing stabilityCompatible with UHPLC systems operating up to 1000 bar
The ZORBAX RRHD range use 1.8 µm particles which allow you to push flow rates to the limits and still achieve high efficiency separations in the shortest run time. By employing a superior packing process these columns are fully stable at operating pressures of up to 1200 bar allowing you to achieve high-throughput separations without compromising on resolution.

ZORBAX RRHD columns increase your productivity with:

  • 1.8 µm particles - deliver maximum resolution for the most defined separations
  • High pressure -1200 bar columns for optimum results with the 1290 Infinity LC or other UHPLC instruments such as Waters Acquity
  • Selectivity and stability – a wide variety of chemically bonded phase are available to provide alternate selectivity at extremes of pH  
  • Scalability - through identical column chemistries the same selectivity can be achieved for easy, fast and secure method transfer
  • Fast or high resolution separations – 50, 100, 150 mm lengths allow fast, efficient separations
 
Agilent HPLC Column Selection Guide Brochure   Zorbax RRHD Flyer follow us on facebookLike Crawford Scientific on Facebook and keep up-to-date with our latest technical tips, offers, promotions, and Chromatography training schedule.

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Agilent HPLC Column Selection Guide   Zorbax RRHD Columns
 

ZORBAX RRHD columns are available in the following chemistries:

ZORBAX RRHD Column Specifications
Bonded Phase Pore Size Surface Area pH Range Endcapped Temp. Limit
ZORBAX Eclipse Plus C18 95Å 160 m2/g 2.0-9.0 Double 60oC
ZORBAX Eclipse Plus C8 95Å 160 m2/g 2.0-9.0 Double 60oC
ZORBAX Eclipse Plus Phenyl-Hexyl 95Å 160 m2/g 2.0-8.0 Double 60oC
ZORBAX Eclipse XDB-C18 80Å 180 m2/g 2.0-9.0 Double 60oC
ZORBAX Extend-C18 80Å 180 m2/g 2.0-11.5† Double 60oC (up to pH8)
40oC (pH8-11.5)
ZORBAX Bonus RP 80Å 180 m2/g 2.0-9.0 Triple 60oC
ZORBAX StableBond SB-C18 80Å 180 m2/g 1.0-8.0* No 90oC
ZORBAX StableBond SB-C8 80Å 180 m2/g 1.0-8.0* No 80oC
ZORBAX StableBond SB-Phenyl 80Å 180 m2/g 1.0-8.0* No 80oC
ZORBAX StableBond SB-CN 80Å 180 m2/g 1.0-8.0* No 80oC
ZORBAX StableBond SB-Aq 80Å 180 m2/g 1.0-8.0* No 80oC
ZORBAX Eclipse PAH 95Å 160 m2/g 1.0-8.0* No 60oC
ZORBAX HILIC Plus 95Å 160 m2/g 2.0-8.0 No 80oC
ZORBAX StableBond 300SB-C8 300Å 45 m2/g 1.0-8.0* No 80oC
ZORBAX StableBond 300SB-C18 300Å 45 m2/g 1.0-8.0* No 90oC
ZORBAX StableBond 300SB-C3 300Å 45 m2/g 1.0-8.0* No 80oC
ZORBAX 300-Diphenyl 300Å 45 m2/g 1.0-8.0* Yes 80oC

* StableBond columns are designed for optimal use at low pH. At pH >6, highest column stability for all silica based columns is obtained by operating at temperatures <40°C and using lower buffer concentrations – 10-20 mM or organic buffers. 300SB-C18 may be used up to 90°C. For pH 6-8, select the Eclipse Plus C18 column.
Temperature limits are 60 °C up to pH 8, 40°C from pH 8-11.5.

 

       Achieve true high-definition    separations without sacrificing stability

Agilent HPLC Column Selection Guide Brochure   Zorbax RRHD Flyer
Agilent HPLC Column Selection Guide   Zorbax RRHD Columns

 

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Recommended for initial method development. These columns provide excellent peak shape for acidic, basic and neutral compounds and are an excellent alternative to Waters Acquity BEH C18/C8 over pH range 2-9.

 

 

  Eclipse Plus C18
(USP L1)

Eclipse Plus C8
(USP L7)
Eclipse Plus
Phenyl-Hexyl
RRHD 2.1 x 150 mm, 1.8 μm
959759-902
959759-906
959759-912
RRHD 2.1 x 100 mm, 1.8 μm
959758-902
959758-906
959758-912
RRHD 2.1 x 50 mm, 1.8 μm
959757-902
959757-906
959757-912
RRHD 3.0 x 150 mm, 1.8 μm
959759-302
959759-306
 
RRHD 3.0 x 100 mm, 1.8 μm
959758-302
959758-306
959758-312
RRHD 3.0 x 50 mm, 1.8 μm
959757-302
959757-306
959757-312

 

seven biocides on a ZORBAX RRHD Eclipse Plus C18

 

Rapid, high resolution separation of seven biocides on a ZORBAX RRHD Eclipse Plus C18 (2.1 x 50 mm, 1.8 µm) - Full experimental details.

Longest column lifetime and best reproducibility for low pH separations. These fully water wettable columns give excellent peak shape for basic compounds. These columns can be used as an alternative to Waters XBridge BEH C18 columns at pH <2.

 

 

  StableBond
SB-C18
(USP L1)
StableBond
SB-C8
(USP L7)
StableBond
SB-Phenyl
(USP L11)
StableBond
SB-CN
(USP L10)
StableBond
SB-Aq
RRHD 2.1 x 150 mm, 1.8 μm  
859700-902
859700-906
859700-912
859700-905
859700-914
RRHD 2.1 x 100 mm, 1.8 μm  
858700-902
858700-906
858700-912
858700-905
858700-914
RRHD 2.1 x 50 mm, 1.8 μm  
857700-902
857700-906
857700-912
857700-905
857700-914
RRHD 3.0 x 150 mm, 1.8 μm  
859700-302
859700-306
     
RRHD 3.0 x 100 mm, 1.8 μm  
858700-302
858700-306
858700-312
858700-305
858700-314
RRHD 3.0 x 50 mm, 1.8 μm  
857700-302
857700-306
857700-312
857700-305
857700-314

 

 Polyphenols on an Agilent ZORBAX RRHD SB-C18

 

Chromatogram and experimental conditions for the separation of nineteen polyphenols on an Agilent ZORBAX RRHD SB-C18 100 mm column - Full experimental details.

Long life at high pH (up to pH 11.5) and fully stable over the entire pH range 2-11.5. An excellent alternative to Waters XBridge BEH C18 at pH >9.

 

 

Extend-C18
(USP L1)
RRHD 2.1 x 150 mm, 1.8 μm
759700-902
RRHD 2.1 x 100 mm, 1.8 μm
758700-902
RRHD 2.1 x 50 mm, 1.8 μm
757700-902
RRHD 3.0 x 150 mm, 1.8 μm
RRHD 3.0 x 100 mm, 1.8 μm
758700-302
RRHD 3.0 x 50 mm, 1.8 μm
757700-302

 

ZORBAX RRHD Extend C18 for the separation of endocannabinoids

 

Selectivity of ZORBAX RRHD Extend C18 (2.1 x 100 mm, 1.8 µm) for the separation of endocannabinoids - Full experimental details.

A polar embedded phase with a very unique selectivity for the retention and separation of polar compounds.  Gives excellent peak shape for challenging basic compounds at both low and mid pH and is an excellent alternative to Waters BEH Shield or HSS T3 phases.

 

 

Bonus RP
RRHD 2.1 x 150 mm, 1.8 μm
859768-901
RRHD 2.1 x 100 mm, 1.8 μm
858768-901
RRHD 2.1 x 50 mm, 1.8 μm
857768-901

 

Separation of Triazine Pesticides on Bonus-RP and Alkyl C8 Phase

 

Separation of Triazine Pesticides on Bonus-RP

 

Agilent ZORBAX Bonus-RP Polar-linked Alkyl Reversed-Phase HPLC Columns 5968-6253 - Full experimental details.

 

Extra dense bonding and double end-capping provides good peak shape for basic, acidic and neutral compounds.

 

 

Eclipse XDB-C18
(USP L1)
RRHD 2.1 x 150 mm, 1.8 μm
981759-902
RRHD 2.1 x 100 mm, 1.8 μm
981758-902
RRHD 2.1 x 50 mm, 1.8 μm
981757-902
RRHD 3.0 x 150 mm, 1.8 μm
981759-302
RRHD 3.0 x 100 mm, 1.8 μm
981758-302
RRHD 3.0 x 50 mm, 1.8 μm
981757-302

 

 

ZORBAX RRHD Extend C18 for the separation of endocannabinoids

 

Selectivity of ZORBAX RRHD Extend C18 (2.1 x 100 mm, 1.8 µm) for the separation of endocannabinoids - Full experimental details.

Provides high resolution separation of PAHs and give excellent peak shapes for basic compounds.

 

 

Eclipse PAH
(USP L1)
RRHD 2.1 x 150 mm, 1.8 μm
959759-918
RRHD 2.1 x 100 mm, 1.8 μm
959758-918
RRHD 2.1 x 50 mm, 1.8 μm
959757-918
RRHD 3.0 x 100 mm, 1.8 μm
959758-318
RRHD 3.0 x 50 mm, 1.8 μm
959757-318

 

Separation of 18 PAH compounds on a ZORBAX RRHD Eclipse PAH C18

 

Separation of 18 PAH compounds on a ZORBAX RRHD Eclipse PAH C18 (2.1 x 100 mm, 1.8 µm) - Full experimental details.

 

Non end-capped for good retention of small, polar analytes.

 

 

HILIC Plus
RRHD 2.1 x 150 mm, 1.8 μm
959759-901
RRHD 2.1 x 100 mm, 1.8 μm
959758-901
RRHD 2.1 x 50 mm, 1.8 μm
959757-901
RRHD 3.0 x 100 mm, 1.8 μm
959758-301
RRHD 3.0 x 50 mm, 1.8 μm
959757-301

 

Optimised LC-MS/MS analysis of vitamin B related compounds separated on a ZORBAX RRHD HILIC Plus

 

Optimised LC-MS/MS analysis of vitamin B related compounds separated on a ZORBAX RRHD HILIC Plus (2.1 x 100 mm, 1.8 µm) - Full experimental details.

 

Provides UHPLC performance for intact proteins and digests.

 

 

StableBond
300SB-C18
StableBond
300SB-C8
StableBond
300SB-C3
300-
Diphenyl
RRHD 2.1 x 100 mm, 1.8 μm
858750-902
858750-906
858750-909
858750-944
RRHD 2.1 x 50 mm, 1.8 μm
857750-902
857750-906
857750-909
857750-944

 

Ultra-fast separation of reduced monoclonal antibodies using a ZORBAX RRHD 300-Diphenyl

 

Ultra-fast separation of reduced monoclonal antibodies using a ZORBAX RRHD 300-Diphenyl (2.1 x 100 mm, 1.8 µm) (a) narrow peak widths and shorter retention times, (b) higher resolution for heavy chain peaks with reduced efficiency compared to (a) - Full experimental details.

 

It is important to protect your UHPLC column from the build up of particulates as this can lead to increased backpressures and shortened column lifetime. Agilent UHPLC guards are high performance guard columns with easy-to-install hardware that fits directly on the end of the column meaning no extra hardware is required.

Ordering Information

 

  Eclipse Plus C18
(USP L1)

Eclipse XDB-C18
(USP L1)
SB-C18
(USP L1)
SB-C8
(USP L7)
RRHD Guard, 1200 bar
2.1 x 5 mm, 1.8 μm
821725-901
821725-903
821725-902
821725-904
RRHD Guard, 1200 bar
3.0 x 5 mm, 1.8 μm
823750-901
823750-903
823750-902
823750-904
RRHT Guard, 600 bar
4.6 x 5 mm, 1.8 μm
820750-901
820750-903
820750-902
820750-904

 

IMPORTANT INFORMATION ABOUT YOUR AGILENT FAST GUARDS FOR UHPLC

Fast Guard User Guide

 

 

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