ZORBAX Rapid Resolution High Definition (RRHD) Columns - Analyse more samples, faster

ZORBAX RRHD columns are available in the following chemistries:

* StableBond columns are designed for optimal use at low pH. At pH >6, highest column stability for all silica based columns is obtained by operating at temperatures <40 °C and using lower buffer concentrations – 10-20 mM or organic buffers. 300SB-C18 may be used up to 90 °C. For pH 6-8, select the Eclipse Plus C18 column.
†Temperature limits are 60 °C up to pH 8, 40 °C from pH 8-11.5.

• Agilent 6530 Accurate-Mass Q-TOF LC MS with Agilent 1290 Infinity and ZORBAX RRHD Eclipse Plus C18 for Multi Plant Growth Regulator Analysis from Grape
• Analyse MAb and BSA digests using ZORBAX RRHD 300SB-C18
• Analysis of impurities in fine chemical octyl-dimethyl-4-aminobenzoate using Agilent 1290 infinity and ZORBAX RRHD SB C18
• Analysis of natural and artificial vanilla preparations using ZORBAX RRHD Eclipse Plus C18
• Analysis of oxidized insulin chains using ZORBAX RRHD 300SB-C18
• Analytical instrument qualification and system validation according to USP Chapter 1058 for the Agilent 1290 Infinity
• Automated Sample Preparation by Protein Precipitation for High Throughput Bioanalysis using ZORBAX RRHD Eclipse Plus C18
• Comparing HILIC and RPLC of Morphine using ZORBAX RRHD columns with UHPLC MS
• Determination of pesticides in baby food by UHPLC MS MS using Agilent 1290 Infinity and 6460 triple quad with ZORBAX RRHD Eclipse Plus C18
• Extended ionization capability of thermal gradient focusing ESI in high throughput in vitro ADME assays ZORBAX RRHD SB C18
• Fast analysis of fat soluble vitamins using the Agilent 1290 Infinity LC and ZORBAX RRHD SB C18
• Fast analysis of resveratrol in red wine using the Agilent 1290 Infinity and ZORBAX RRHD SB C18
• Fast LC MS MS Analysis of Group 4 Pharmaceuticals from EPA-1694 with ZORBAX RRHD HILIC Plus
• Fast Separation of Biocides Using ZORBAX RRHD Eclipse Plus C18 5990-4899EN
• Fast Separation of Recombinant Human Erythropoietin Using ZORBAX RRHD 300SB-C18
• Fully Using High Efficiency Columns with LCMS and ZORBAX RRHD Eclipse Plus C18
• High resolution of complex lipids using the Agilent 1290 Infinity LC and ZORBAX RRHD columns
• High speed separation of anesthetics on the Agilent 1290 Infinity with ZORBAX RRHD Eclipse Plus C18 and Waters BEH
• High throughput bioanalytical method development using UHPLC_triple quad MS and ZORBAX RRHD Eclipse Plus C18
• High-Resolution Analysis of Intact Triglycerides using the Agilent 1290 Infinity and ZORBAX Eclipse Plus SB C18
• HILIC MS MS of Morphine and Metabolites with an Ultra Low Dispersion Agilent 1290 LC and ZORBAX RRHD HILIC Plus
• Improved mass accuracy by enhanced separation of compounds of isobaric mass using Agilent 1290 Infinity and ZORBAX RRHD Eclipse Plus C18
• Increasing Resolution and Speed by Operating UHPLC columns up to 1200 bar
• Investigating organic contamination in high purity water using ZORBAX RRHD SB C18 with ultrasensitive diode array detection and LC MS
• LC MS MS of Vitamin B Shows Effects of Injection Solvents with ZORBAX RRHD HILIC Plus 5991-0209EN
• Optimizing Performance of ZORBAX RRHD Eclipse Plus C18 by enhancing an Agilent 1290 Infinity System for Ultra-Low Dispersion
• Pesticide Analysis using Agilent 1290 Infinity with an Agilent 6140 Single Quad LCMS and ZORBAX RRHD Eclipse plus C18
• Rapid Method Development for 18 PAHs ZORBAX RRHD Eclipse PAH 5990-8432EN
• Rapid UHPLC Analysis of Reduced MAbs using ZORBAX RRHD 300SB-C8
• Reversed-Phase Optimization for Ultra Fast Profiling of Intact and Reduced MAbs using ZORBAX RRHD 300SB-C3
• Reversed-phase Separation of Intact MAbs using ZORBAX RRHD 300SB-C8
• Scalability of Agilent columns across HPLC and UHPLC instruments
• Screening Impurities in Fine Chemicals Using the Agilent 1290 Infinity LC and ZORBAX RRHD Eclipse Plus C18N
• Several ZORBAX RRHD Selectivities Facilitate Method Development 5990-7166EN
• Speed up Analysis of Notoginsenoside R1 and Ginsenosides Rg1 Re and Rb1 in compound TCM using the 1290 Infinity and ZORBAX RRHD
• UHPLC of polyphenols in wine using ZORBAX RRHD SB C-18 5990-5659EN
• Ultra High Speed and High Res Separations of Reduced and Intact MAbs with ZORBAX RRHD 300 Diphenyl 5990-9668EN
• Ultrafast analysis of synthetic antioxidants in vegetable oils using ZORBAX RRHD Eclipse Plus C18 and the Agilent 1290 Infinity
• Ultrafast, sensitive analysis of sweeteners, preservatives and flavorants in nonalcoholic beverages using ZORBAX RRHD Eclipse Plus C18 and the Agilent 1290 Infinity
• ZORBAX RRHD 300SB-C18 for the analysis of proteins
• ZORBAX RRHD Phases Offers Selectivity Options for the Determination of Anthocyanins in Blueberries with UHPLC MS
• Agilent ZORBAX Bonus-RP Polar-linked Alkyl Reversed-Phase HPLC Columns 5968-6253 - Full experimental details.

Agilent HPLC Column Selection Guide		Zorbax RRHD Columns

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Recommended for initial method development. These columns provide excellent peak shape for acidic, basic and neutral compounds and are an excellent alternative to Waters Acquity BEH C18/C8 over pH range 2-9.

Rapid, high resolution separation of seven biocides on a ZORBAX RRHD Eclipse Plus C18 (2.1 x 50 mm, 1.8 µm) - Full experimental details.

Longest column lifetime and best reproducibility for low pH separations. These fully water wettable columns give excellent peak shape for basic compounds. These columns can be used as an alternative to Waters XBridge BEH C18 columns at pH <2.

Chromatogram and experimental conditions for the separation of nineteen polyphenols on an Agilent ZORBAX RRHD SB-C18 100 mm column - Full experimental details.

Long life at high pH (up to pH 11.5) and fully stable over the entire pH range 2-11.5. An excellent alternative to Waters XBridge BEH C18 at pH >9.

Selectivity of ZORBAX RRHD Extend C18 (2.1 x 100 mm, 1.8 µm) for the separation of endocannabinoids - Full experimental details.

A polar embedded phase with a very unique selectivity for the retention and separation of polar compounds.  Gives excellent peak shape for challenging basic compounds at both low and mid pH and is an excellent alternative to Waters BEH Shield or HSS T3 phases.

Separation of Triazine Pesticides on Bonus-RP and Alkyl C8 Phase

Agilent ZORBAX Bonus-RP Polar-linked Alkyl Reversed-Phase HPLC Columns 5968-6253 - Full experimental details.

Extra dense bonding and double end-capping provides good peak shape for basic, acidic and neutral compounds.

Selectivity of ZORBAX RRHD Extend C18 (2.1 x 100 mm, 1.8 µm) for the separation of endocannabinoids - Full experimental details.

Provides high resolution separation of PAHs and give excellent peak shapes for basic compounds.

Separation of 18 PAH compounds on a ZORBAX RRHD Eclipse PAH C18 (2.1 x 100 mm, 1.8 µm) - Full experimental details.

Non end-capped for good retention of small, polar analytes.

Optimised LC-MS/MS analysis of vitamin B related compounds separated on a ZORBAX RRHD HILIC Plus (2.1 x 100 mm, 1.8 µm) - Full experimental details.

Provides UHPLC performance for intact proteins and digests.

Ultra-fast separation of reduced monoclonal antibodies using a ZORBAX RRHD 300-Diphenyl (2.1 x 100 mm, 1.8 µm) (a) narrow peak widths and shorter retention times, (b) higher resolution for heavy chain peaks with reduced efficiency compared to (a) - Full experimental details.

It is important to protect your UHPLC column from the build up of particulates as this can lead to increased backpressures and shortened column lifetime. Agilent UHPLC guards are high performance guard columns with easy-to-install hardware that fits directly on the end of the column meaning no extra hardware is required.

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IMPORTANT INFORMATION ABOUT YOUR AGILENT FAST GUARDS FOR UHPLC

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