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ZORBAX Eclipse Plus HPLC Columns

Agilent Technologies ZORBAX Eclipse Plus HPLC Columns  

Eclipse Plus (C18, C8, Phenyl-Hexyl and PAH) columns provide the ultimate in performance for silica-based columns. Peak shape is excellent for the most challenging basic compounds, improving efficiency and resolution with these sample types. These results are achieved by improvements in the silica manufacturing and bonding technology, which is completely controlled by Agilent. Because of their high level of performance, Eclipse Plus columns are the ideal first choice for method development of all samples. To achieve fast method development and superior productivity, choose a column with high-resolution 1.8 µm particles.
The ZORBAX Rapid Resolution High Definition Columns are available in Eclipse Plus C18.
For standard methods, conventional 5 µm and Rapid Resolution 3.5 µm columns are your best choice. With all particle sizes, easy method transfer is possible.

 
  • Excellent peak shape for basic compounds
  • High level of performance—peak shape, efficiency, resolution, and lifetime—with all sample types: acids, bases and neutrals
  • Multiple selectivity choices (C18, C8, PAH and Phenyl-Hexyl) for optimized resolution of all sample types
  • Superior reproducibility with more rigorous QA/QC testing
  • Improved, patented silica manufacturing with start-to-finish product control
  • Available in 1.8, 3.5 and 5 µm particle sizes for all analytical, high resolution, and fast LC analyses
  • Available in C18, C8 new Phenyl-Hexyl and a C18 for PAH separations
 
Eclipse Plus columns are recommended for initial method development from pH 2-9 and provide high resolution and excellent peak shape of all types of compounds. Like all Eclipse columns Eclipse Plus columns achieve superior performance through extra dense bonding and a precise double-endcapping process. But Eclipse Plus columns also feature enhanced high-performance silica, new endcapping reagents, and a unique bonding process that is optimized for the improved silica.
     

C18 and C8 Eclipse Plus columns are available in the following particle sizes:

     
     

ZORBAX Eclipse Plus Analytical HPLC Columns

This is the particle size for almost all standard analytical HPLC assays.
For full details of the range of analytical columns available please see below.
     
ZORBAX Eclipse Plus Comparison Chromatograms
     
Description Size (mm) Particle Size (μm) Eclipse Plus C18
USP L1
Eclipse Plus C8
USP L7
         
Analytical 4.6 x 250 5
959990-902
959990-906
Analytical 4.6 x 150 5
959993-902
959993-906
Analytical 4.6 x 100 5
959996-902
959996-906
Analytical 4.6 x 50 5
959946-902
959946-906
Solvent Saver 3.0 x 150 5
959993-302
959993-306
Narrow Bore 2.1 x 150 5
959701-902
959701-906
Narrow Bore RR 2.1 x 50 5
959746-902
959746-906
Guard Cartridges, 4/pk 4.6 x 12.5 5
820950-936
820950-937
Guard Cartridges, 4/pk 2.1 x 12.5 5
821125-936
821125-937
     

ZORBAX Eclipse Plus Rapid Resolution HPLC Columns

  • Reduce analysis time and solvent usage and increase sample throughput
  • High efficiency in short and ultra-short column lengths
  • Available in analytical (4.6 mm) and narrow-bore (2.1 mm) ID
  • Comparable lifetime to 5 μm columns
     
Agilent ZORBAX Rapid Resolution columns, with a 3.5 μm particle size, reduce analysis time and increase sample throughput for any application when compared to 5 μm columns.
Rapid Resolution columns are available from 15-150 mm, in 1-4.6 mm IDs, so the best configurations are available for high throughput, LC/MS, combinatorial chemistry and rapid analytical applications.
Rapid Resolution 3.5 μm particles have superior mechanical strength, so every column has an extremely stable packed bed and provides a comparable lifetime to 5 μm columns.
     
Eclipse Plus C18 Separation of Histamines
     
Description Size (mm)  ParticleSize (μm)  EclipsePlus C18 USP L1 EclipsePlus C8 USP L7
         
Rapid Resolution 4.6 x 150 3.5
959963-902
959963-906
Rapid Resolution 4.6 x 100 3.5
959961-902
959961-906
Rapid Resolution 4.6 x 75 3.5
959933-902
959933-906
Rapid Resolution 4.6 x 50 3.5
959943-902
959943-906
Rapid Resolution 4.6 x 30 3.5
959936-902
959936-906
Solvent Saver Plus 3.0 x 150 3.5
959963-302
959963-306
Solvent Saver Plus 3.0 x 100 3.5
959961-302
959961-306
Narrow Bore RR 2.1 x 150 3.5
959763-902
959763-906
Narrow Bore RR 2.1 x 100 3.5
959793-902
959793-906
Narrow Bore RR 2.1 x 50 3.5
959743-902
959743-906
Narrow Bore RR 2.1 x 30 3.5
959733-902
959733-906
     

ZORBAX Eclipse Plus Rapid Resolution High Throughput HPLC Columns

  • New high pressure (600 bar) columns for ultra high speed or maximum resolution analyses with Rapid Resolution HT columns packed with totally porous, 1.8 μm packings
  • Carefully engineered particles deliver maximum resolution at 25% less pressure than other sub 2-micron materials
  • Reduce analysis time by up to 95%
  • Develop HPLC methods more quickly
  • Securely transfer conventional methods with over 80 RRHT column choices
  • Analyze complex samples on shorter columns faster and maximize peak capacity
  • Perform faster analyses and use less solvent
  • Short (50 mm long and less) column can be used on some conventional LCs
     
Eclipse Plus RRHT Separation of Berberine
     
ZORBAX Rapid Resolution HT (1.8 μm) columns use a totally porous, 1.8 μm particle to provide maximum resolution in fast, ultra-fast and high resolution analyses. You can reduce analysis time by up to 95% in comparison to 250 mm length columns.
The small particle size provides double the efficiency of a 3.5 μm column in the same column length providing the highest efficiency and resolution possible. This permits the analysis of complex samples on shorter column with the highest resolution and peak capacity.
The 1.8 μm Rapid Resolution HT columns take high-speed, high-resolution HPLC to a new level. The 600 bar columns can be used with the new Agilent 1200 Rapid Resolution LC up to this high pressure limit. In addition, the shorter columns can be used on many other LC's, including the Agilent 1100 by using the RRHT-1100 conversion kits to maximize performance.
     
Description Size (mm) 
ParticleSize (μm) 
EclipsePlus C18 USP L1 EclipsePlus C8 USP L7
   
   
Rapid Resolution HT, 600 bar 4.6 x 150
1.8
959994-902
 
Rapid Resolution HT, 600 bar 4.6 x 100
1.8
959964-902
959964-906
Rapid Resolution HT, 600 bar 4.6 x 50
1.8
959941-902
959941-906
Rapid Resolution HT, 600 bar 4.6 x 30
1.8
959931-902
959931-906
Solvent Saver HT, 600 bar 3.0 x 100
1.8
959964-302
959964-306
Solvent Saver HT, 600 bar 3.0 x 50
1.8
959941-302
959941-306
Narrow Bore RRHT, 600 bar 2.1 x 100
1.8
959764-902
959764-906
Narrow Bore RRHT, 600 bar 2.1 x 50
1.8
959741-902
959741-906
Narrow Bore RRHT, 600 bar 2.1 x 30
1.8
959731-902
959731-906
     

ZORBAX Eclipse Plus Rapid Resolution High Definition HPLC Columns

  • High pressure (1200 bar) columns for optimum results with the 1290 Infinity LC or other UHPLC instruments
  • 1.8 µm particles deliver maximum resolution for the most defined separations
  • Achieve the same selectivity on the 3.5 and 5 µm ZORBAX columns with the same bonded phase for compatibility with any LC
 
For use up to 1200 bar ZORBAX Rapid Resolution High Definition (RRHD) columns are an expansion of the ZORBAX 1.8 µm particle column line. The new RRHD columns use improved packing processes to achieve stability up to 1200 bar for use with the Agilent 1290 Infinity LC. The RRHD 1.8 µm columns are available in 50, 100 and 150 mm lengths for fast or high resolution – truly high definition – separations of your most complex samples.
     
Description Size (mm) 
ParticleSize (μm) 
EclipsePlus C18 USP L1
   
 
Rapid Resolution HD, 1200 bar 4.6 x 100
1.8
959758-902
Rapid Resolution HD, 1200 bar 4.6 x 50
1.8
959757-902
     
 
 
 


 

 

 

 

 

 

 

 

 

 

 

 

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