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in home > Products > Manufacturers > Agilent Technologies > HPLC Columns > ZORBAX Normal Phase |
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ZORBAX Original Normal Phase HPLC Columns |
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For normal-phase chromatography, the Agilent ZORBAX product line offers a choice of
bonded and non-bonded silica packings.
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ZORBAX Rx-SIL
- Made from highly pure (>99.995%) porous silica microspheres (pore size is the space
between the solid silica microparticles)
- Available in 1.8 and 5 μm particle sizes
- Stronger than other silica types
- Less acidic than ZORBAX-SIL, lower metal content
- Low acidity and low metal content make ZORBAX Rx-SIL ideal for normal-phase separation
of polar compounds that exhibit poor peak symmetry on more acidic silica
- Useful for very hydrophilic compounds with high organic mobile phases in HILIC mode
ZORBAX Eclipse XDB-CN
- Made from highly pure Rx-SIL
- Excellent choice for normal phase applications with basic compounds
- Equilibrates more rapidly than ZORBAX Rx-SIL and is used for many of the same
normal-phase applications
ZORBAX CN
- Cyanopropyldimethylsilane monolayer bonded to ZORBAX SIL
- Equilibrates more rapidly than ZORBAX SIL, and used for many of the same
normal-phase applications
- Less prone to fouling and less water sensitive than silica
ZORBAX NH2
- Amino-propyl silane phase bonded to ZORBAX SIL
- Used for normal phase and weak anion-exchange, and reversed-phase HPLC of polar compounds
- Vitamins A and D are separated in the normal-phase mode
- Carbohydrates and sugars are separated in the reversed-phase mode
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Size (mm) |
SIL
USP L3
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CN
USP L10
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NH2
USP L8 |
Carbohydrate
Analysis * |
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| Standard Columns (no special hardware required) |
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| Semi-Prep 5µm |
9.4 x 250 |
880952-201 |
880952-205 |
880952-208 |
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| Analytical |
4.6 x 250 |
880952-701 |
880952-705 |
880952-708 |
840300-908 |
| Analytical |
4.6 x 150 |
883952-701 |
883952-705 |
883952-708 |
843300-908 |
| Narrow Bore |
2.1 x 50 |
860700-708 |
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| Guard Columns (hardware required) |
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| Guard Cartridge, 2/pk |
9.4 x 15 |
820675-119 |
820675-111 |
820675-111 |
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| Guard Cartridges, 4/pk |
4.6 x 12.5 |
820950-901 |
820950-905 |
820950-908 |
820950-908 |
| Guard Hardware Kit |
9.4 mm |
840140-901 |
840140-901 |
840140-901 |
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| Guard Hardware Kit |
4.6 mm |
820888-901 |
820888-901 |
820888-901 |
820888-901 |
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| PrepHT Cartridge Columns (require endfittings kit 820400-901) |
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| PrepHT Cartridge |
21.2 x 250 |
877952-101 |
877952-105 |
877952-108 |
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| PrepHT endfittings, 2/pk |
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820400-901 |
820400-901 |
820400-901 |
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| *These columns ship containing reversed phase solvents. Flush with isopropanol before using normal phase solvents. These columns can also be used in HILIC mode. |
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| Bonded Phase |
Pore Size |
Surface Area |
pH Range |
Endcapped |
Carbon Load |
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| ZORBAX Rx-SIL |
80Å |
180 m2/g |
0 - 8.0 |
No |
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| ZORBAX Eclipse XDB-CN |
80Å |
180 m2/g |
2.0 - 8.0 |
Yes |
4.3% |
| ZORBAX SIL |
70Å |
300 m2/g |
0 - 8.0 |
No |
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| ZORBAX CN |
70Å |
300 m2/g |
2.0 - 7.0 |
Yes |
7% |
| ZORBAX NH2 |
70Å |
300 m2/g |
2.0 - 7.0 |
Yes |
4% |
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