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ZORBAX Original Normal Phase HPLC Columns

Zorbax Normal Phase HPLC  

For normal-phase chromatography, the Agilent ZORBAX product line offers a choice of bonded and non-bonded silica packings.

ZORBAX Rx-SIL

  • Made from highly pure (>99.995%) porous silica microspheres (pore size is the space
    between the solid silica microparticles)
  • Available in 1.8 and 5 μm particle sizes
  • Stronger than other silica types
  • Less acidic than ZORBAX-SIL, lower metal content
  • Low acidity and low metal content make ZORBAX Rx-SIL ideal for normal-phase separation
    of polar compounds that exhibit poor peak symmetry on more acidic silica
  • Useful for very hydrophilic compounds with high organic mobile phases in HILIC mode


ZORBAX Eclipse XDB-CN

  • Made from highly pure Rx-SIL
  • Excellent choice for normal phase applications with basic compounds
  • Equilibrates more rapidly than ZORBAX Rx-SIL and is used for many of the same
    normal-phase applications

ZORBAX CN

  • Cyanopropyldimethylsilane monolayer bonded to ZORBAX SIL
  • Equilibrates more rapidly than ZORBAX SIL, and used for many of the same
    normal-phase applications
  • Less prone to fouling and less water sensitive than silica


ZORBAX NH2

  • Amino-propyl silane phase bonded to ZORBAX SIL
  • Used for normal phase and weak anion-exchange, and reversed-phase HPLC of polar compounds
  • Vitamins A and D are separated in the normal-phase mode
  • Carbohydrates and sugars are separated in the reversed-phase mode



   
Description
5µm columns
Size (mm)
SIL
USP L3

CN
USP L10
NH2
USP L8
Carbohydrate
Analysis *
 
Standard Columns (no special hardware required)
           
Semi-Prep 5µm 9.4 x 250
880952-201
880952-205
880952-208
 
Analytical 4.6 x 250
880952-701
880952-705
880952-708
840300-908
Analytical 4.6 x 150
883952-701
883952-705
883952-708
843300-908
Narrow Bore 2.1 x 50
860700-708
     
 
Guard Columns (hardware required)
           
Guard Cartridge, 2/pk 9.4 x 15
820675-119
820675-111
820675-111
 
Guard Cartridges, 4/pk 4.6 x 12.5
820950-901
820950-905
820950-908
820950-908
Guard Hardware Kit 9.4 mm
840140-901
840140-901
840140-901
 
Guard Hardware Kit 4.6 mm
820888-901
820888-901
820888-901
820888-901
 
PrepHT Cartridge Columns (require endfittings kit 820400-901)
           
PrepHT Cartridge 21.2 x 250
877952-101
877952-105
877952-108
 
PrepHT endfittings, 2/pk  
820400-901
820400-901
820400-901
 
*These columns ship containing reversed phase solvents. Flush with isopropanol before using normal phase solvents. These columns can also be used in HILIC mode.


     
 
Bonded Phase Pore Size   Surface Area  pH Range Endcapped Carbon Load
           
ZORBAX Rx-SIL 80Å 180 m2/g 0 - 8.0 No  
ZORBAX Eclipse XDB-CN 80Å 180 m2/g 2.0 - 8.0 Yes 4.3%
ZORBAX SIL 70Å 300 m2/g 0 - 8.0 No  
ZORBAX CN 70Å 300 m2/g 2.0 - 7.0 Yes 7%
ZORBAX NH2 70Å 300 m2/g 2.0 - 7.0 Yes 4%
 
 
 


 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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