The Agilent Zorbax HPLC column range is one if the most highly regarded and trusted HPLC column brands on the market. Agilent Zorbax Silica columns are the only one of the main manufacturers that can offer you true scalability due to their patented silica manufacturing and ensure consistment batch-to-batch reproducibility.
The largest portfolio of Fast LC columns with a broad range of phases across all particle sizes for exceptional flexibility and scalability. Whether you are performing conventional or ultra-fast chromatography, separating biomolecules, or analysing complex basic compounds, you can trust Agilent for the industry’s highest performing columns that deliver the fast, reproducible results you need. This selection guide is focused on Agilent's small molecule HPLC columns including; Poroshell 120– these columns provide high efficiency and high resolution separations with up to 50% less back-pressure compared to sub-2 μm columns. ZORBAX Rapid Resolution High Definition (RRHD)– these 1.8 μm particle columns feature an improved packing processes to achieve stability up to 1200 bar allowing them to be used with the Agilent 1290 Infinity and other UPLC instruments. ZORBAX Rapid Resolution HighThroughput (RRHT) columns have 1.8 μm particles and are available in 2.1, 3.0 and 4.6 mm I.Ds with 600 bar stability. Download the Agilent HPLC Column Selection Guide for more details and ordering information or alternatively, visit www.crawfordscientific.com/HPLC.htm for all HPLC columns and accessories available from Crawford Scientific.
Poroshell 120 columns provide exceptional efficiency for standard HPLC systems and significantly boost the performance from UHPLC instruments.
These next-generation columns take the technology to the next level with Poroshell 300– giving you higher throughput and resolution for a wide range
of small molecules, peptides and proteins. Their advanced features include:
Comparable speed and resolution to sub-2 μm columns with up to 50% less backpressure
Superior peak shape for faster, more accurate results
Long column life – Poroshell 120 columns use a standard 2 μm frit, and resist
plugging with dirty samples
A growing family of bonded phases for method development flexibility
Easy method transfer and scalability to ZORBAX bonded phases
UHPLC guard column options that reduce your operating expenses by extending
the life of Poroshell 120 columns
Biocolumns are liquid chromatography columns used for the separation of biological compounds such as peptides, proteins, oligonucleotides and other biopolymers.
Biocolumns are specifically designed
for biomolecule analysis with larger pore sizes to accommodate the larger molecules. To improve recovery, the column media
is designed to minimise non-specific binding of analytes. Separation mechanisms are chosen to either retain biological function so bioactivity is not lost during
analysis or to deliberately denature for primary structure characterisation.
Typically, HPLC has been used to separate biomolecules however, advanced techniques such as UHPLC are becoming a popular choice for their characterisation. As a result, Agilent offers advanced chemistries, developed specifically for the separation of biomolecules using size exclusion, reversed-phase, ion-exchange and affinity functionalities all of which are covered in this selection guide.
Agilent CrossLab offers a growing portfolio of HPLC supplies manufactured for seamless performance with a variety of non-Agilent analytical instruments.
Other manufacturers instruments currently supported are Waters, Shimadzu, Dionex (Thermo Scientific) and CTC Analytics.
The growing portfolio of products include:
• Autosampler syringes
• Capillaries, tubing, and fittings
• Detector lamps
• Performance Maintenance kits
• Pump supplies
• Sample loops
• Valve supplies
• Vials and closures
• Well plates and sealing mats
Agilent Technologies is confident in the quality of its ZORBAX HPLC column range. As a result, Crawford Scientific is delighted to offer its customers the option to buy any Zorbax column and return it (within 30 days of shipment) if it does not meet your requirements, to receive a full refund.
Please quote 8642 when placing your order (exclusions apply, please contact us for more information).
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Technical Help
At Crawford Scientific we support you all the way, from the purchase of your column to the establishment of a working HPLC protocol.
Our technical support specialists are here to help you whether it's choosing the correct HPLC column to suit your application or advice to help you develop/troubleshoot a method.
