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Capillary Electrophoresis class room course
1 Day Course | GC Level 1

Capillary electrophoresis (CE) is capable of separating ionic species based on their size to charge, hydrodynamic radius and frictional forces under an electric field. The selection of capillaries, additives and operating conditions are all fully explained, so that working with and troubleshooting CE separations will be much simpler and effective.

The course comprehensively covers the instrumentation and application of CE for electrically driven separations and shows how each mode might be optimized to obtain highest resolution and sensitivity.

  • We limit numbers to 20 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
  • When delivered on-site we can design the course material to suit your specific training needs
  • Customisable written assessments are available if required

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The course comprehensively covers the instrumentation and application of CE for electrically driven separations and shows how each ‘mode’ might be optimized to obtain highest resolution and sensitivity.

Who is this course for

This course is for analysts or method developers who work with CE; or chromatographers and life scientists who are interested in this powerful separation technique.

Previous knowledge

Good knowledge of Chemistry and Physics is necessary. Previous experience using dissolution equipment will be beneficial.

What you will learn

  • The basics of CE
  • Capillary Technology, Electrochromatography and related techniques.
  • Applications in various fields of the Life Sciences
  • Troubleshooting

Basic Concepts

  • Electrical conduction in fluid solution
  • Electroendoosmosis
  • Joule heating
  • Optimizing the voltage and temperature
  • Capillary diameter and buffer ionic strength
  • Temperature effects

Capillary Electrochromatography

  • Modes of CEC
  • Electroosmotic flow in CEC
  • CEC microfluidic devices

Method Development

  • Mobility
  • Solute-wall interactions
  • Separation strategies

 

Injection

  • Injection techniques
  • Injection artefacts: Problems and Solutions
  • Stacking and trace enrichment

Secondary Equilibrium, Micelles,
Cyclodextrins, and Related Reagents

  • Micelles
  • Selecting the electrolyte system
  • Elution range of MECC
  • Chiral recognition
  • Affinity capillary electrophoresis

Detection

  • Detection techniques
  • Band broadening
  • Derivatisation
  • Mass Spectrometry
  • Micropreparative Fraction Collection

Capillary Isoelectric Focusing

  • pH gradient formation
  • Electrode buffer solutions
  • Resolving power
  • Salt effects

Putting it All Together

  • Requirements for robust separations
  • Quantitative analysis
  • Validation
  • Troubleshooting

Training Calendar

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