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Practical Fast HPLC / UHPLC Method Development practical course
2 Day Course | HPLC Level 4

This course is designed as a comprehensive introduction to the principles and practice of Fast HPLC and UHPLC method development.

The course is a mix of theory, computer simulation and laboratory-based practical sessions that allow the student to quickly gain knowledge on the theory, practice and limitations of the common approaches to speeding up HPLC / UHPLC analysis.

The main factors in speeding up analysis - reduced column dimension, sub-2μm particles, core-shell type particles, all combined with elevated temperature and pressure - are discussed in detail and method conversions are practically demonstrated.

  • We limit numbers to 6 per course so that each delegate gets the opportunity to ask questions and fully participate in practical exercises
  • When delivered on-site we can design the course material to suit your specific training needs
  • Customisable written assessments are available if required

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download course pdfThe overall aim of the training is to enable clients to speed up existing separations or to develop novel ‘Fast’ HPLC or UHPLC separations.

Instrumentation is taken into account, as are the various approaches to Fast HPLC using conventional pressure as well as Ultra High Pressure system hardware. The importance of extra-column volume and hysteresis effects are also discussed and demonstrated.

Who is this course for

This course is designed for experienced chromatographers and / or method developers with experience in conventional HPLC, or those HPLC users who are about to transition to fast LC particles or UHPLC instrumentation.

Previous knowledge

Delegates should have a good knowledge of chromatography and experience as HPLC users. Some experience in method development is recommended. A good grounding in chemistry is also beneficial.

What you will learn

  • Why Fast HPLC?
  • So what happened to selectivity?
  • Effects of high pressure on solvent and column properties
  • Transferring methods from HPLC and developing methods for Fast LC from scratch
  • Optimisation strategies


  • Acronyms and nomenclature
  • Theory of Fast and Ultra-fast HPLC
  • Why Fast HPLC: separation speed as a limiting factor in analysis

Solvent, Column and Instrumentation Considerations

  • The influence of column geometry
  • Importance of temperature control
  • Temperature and flow rate interdependence
  • Instrument requirements (delay volume, extra column volume, acquisition rates)


  • Optimising efficiency
  • Optimising plate number: different theories and approaches
  • Superficially porous silica
  • Sub-2μm silica
  • Monolithic silica
  • Effectively measuring plate number
  • Reduced plate heights/Poppe plots

Transferring & Developing Methods

  • Simple software tools for scaling separations
  • Sub-2μm method transfer and optimisation
  • Superficially porous silica method transfer and optimisation
  • Test sample optimisation

Training Calendar

Click on a title below to download a detailed course description or click a date and book your course.

Can't find a suitable training course? Call 01357 522 961 or email us.

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Contact a member of the Crawford Scientific Training team by completing the form below.


HPLC Method Conversion

There are a number of simple equations which can assist with translating HPLC methods to faster equivalents and some of these are explained alongside the simple method translator below.

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