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Solid Phase Extraction class room course
1 Day Course

A must for all users of Solid Phase Extraction (SPE) as a sample preparation technique. You will learn the absolute essentials of SPE and, using our unique multi-media examples and tutorial exercises, you will begin to use your new knowledge and ideas right away.

  • We limit numbers to 20 per course so that each delegate gets the opportunity to ask questions and fully participate in tutorial exercises
  • When delivered on-site we can design the course material to suit your specific training needs
  • Customisable written assessments are available if required

Here's a quick video from one of our technical experts Colin Towers, explaining the basics of SPE.

off site Solid Phase Extraction
on site
on site

A comprehensive one day course designed to increase expertise and optimise method development time for all users of solid phase extraction as a means of sample preparation.

The course is backed with a host of explanatory images and multimedia material from our range of e-learning products. Practical examples are included to reinforce the learning experience.

Who is this course for

This course is for LC or GC analysts or method developers who need to master the principles of Solid Phase Extraction in order to design and implement effective SPE protocols.

Previous knowledge

Knowledge of chromatography and a basic grounding in chemistry are beneficial. Previous experience using SPE will be also be advantageous.

What you will learn

  • The basics of SPE and practical considerations
  • Sorbent Selection
  • What are the various extraction techniques offered by SPE
  • Method development and protocol troubleshooting

SPE Sorbent Substrates

Protocol Steps in SPE

  • SPE Sample Pre-treatment
  • SPE Column Conditioning
  • SPE Column Equilibration
  • Sample Loading
  • Column Washing
  • Analyte Elution

Molecular Properties

  • Hydrophobic or Non-Polar Groups
  • Polar Groups
  • Ionic Groups
  • Chelating Groups

SPE Mechanisms

  • Non-polar SPE
  • Polar SPE
  • Cation-Exchange SPE
  • Anion-Exchange SPE
  • Mixed-Mode SPE

SPE Method Development

  • Analyte Assessment
  • Mechanism Selection
  • Sorbent Screening
  • Procedure Optimization

Training Calendar

Click on a title below to download a detailed course description or click a date and book your course.

Can't find a suitable training course? Call 01357 522 961 or email us.

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Contact a member of the Crawford Scientific Training team by completing the form below.


Polar SPE

Polar SPE is the extraction process where molecules with polar functional groups are extracted from predominantly non-polar matrices onto sorbents exhibiting polar surface properties.

In polar SPE, the retention mechanism is the interaction of polar groups on the analytes of interest and polar functional groups on the sorbent, typically via dipole-dipole or hydrogen-bonding interactions. These interactions are disrupted (causing elution of the analytes) by solvents with significant polar character (i.e., containing dipoles and/or heteroatoms, especially polar oxygen or nitrogen atoms). This interaction is facilitated (allowing retention of the analytes) by solvents having very little polar character; or, in other words, very non-polar solvents.

Most pharmaceutical biological samples are aqueous in nature. Water is a very poor solvent in which to begin a polar SPE protocol, since by definition water is extremely polar, making it a very strong elution solvent for polar extractions. Therefore, analytes applied to polar SPE columns directly from aqueous matrices tend to breakthrough the sorbent bed. An ideal initial step for polar SPE of biological samples is a liquid/liquid extraction into an appropriate non-polar solvent.
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Examples of common sorbents used in polar SPE include diol, aminopropyl, propylcyano, unbonded silica, alumina and Florisil™. Less common but nevertheless quite useful are ion-exchangers, which do not function as ionic sorbents in non-polar solvent environments, but often have considerable polar character in a non-polar organic solvent. Other than alumina and Florisil™, most phases used for polar extractions are silica-based — polymer-based polar sorbents are rare.
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