High Performance Liquid Chromatography

Fundamental Liquid Chromatography
HPLC Troubleshooting & Maintenance
HPLC Method Development
2 day Method Development with Computer Simulation Exercises

Each of our Liquid Chromatography courses are supplemented with full colour delegate handbooks as well as tutorial exercises and interactive multi-media from our elearning website www.CHROMacademy.com - in association with LCGC.

Each course may be customised to your specific business and analytical requirements.

Each of these courses can be assessed if required

For more information on these courses or to download our training brochure – click here

Module 1 - Fundamentals of HPLC

Basic Principles of the Chromatographic Process
Overview of System Components
Main mechanisms of Chromatography and Principles of Ionisation
Sample Preparation Protocols
Sample Introduction - Principles, Choice of Technique and Operation
Column Theory and Stationary Phase Considerations
Detectors - Choice and Operation (including UV, RI, fluorescence)
Measuring and Optimising Chromatographic Parameters (R, N, a , k)
Basic Maintenance and Troubleshooting
Methods of Quantitation

Module 2 - HPLC Troubleshooting and Maintenance

(To include symptoms, causes, diagnosis and solution in each case)

Approaches to Troubleshooting - a discussion
Hardware Troubleshooting and Maintenance (solvent, sample, temperature and pressure control, injection systems, column and detector systems)
Chromatographic Troubleshooting (based on chromatographic parameters and the chromatogram - baseline problems, peak shape, retention stability etc.)
Problems with quantitation
System records

Module 3 - HPLC Method Development

All our method development courses are designed in conjunction with our clients to ensure maximum relevance. All include simulated method developments to highlight critical issues and concepts.

Strategies and Implementation Protocols
Existing Information - what is known, what should be known
Separation Processes
adsorption chromatography, reverse phase, ion suppression, ion exchange, gel permeation, gel filtration, affinity chromatography
Sample Preparation Protocols
Choice and Optimisation of Detection Technique
Optimisation of Chromatographic Parameters - a practical approach
System Suitability Testing
Method Validation - an overview

Module 4 – 2 day Method Development with Computer Simulation Exercises

These are available for inclusion in the above courses on request. Experiments are available to practically reinforce concepts that are covered in the various modules. The details of these can be arranged with the training sponsor to cover:

The Effect of pH on basic acidic and neutral species
Gradient optimisation, including Final %B and gradient Steepness
Use of the K* equation
Influence of Shape Selectivity
Calculation a suitable isocratic composition
Determination of Chromatographic Parameters
Column parameters; Particle size and flow rate effects
Treatment of the Van Deemter equation

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Training brochure

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