Chromatography Consumables, Analytical Services and Chromatography / Mass Spectrometry Training from Crawford Scientific
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Gas Chromatography
 


 		 Fundamental Gas Chromatography
GC Troubleshooting & Maintenance
GC Method Development
Practical Exercises

Each of our Gas Chromatography courses are supplemented with full colour delegate handbooks as well as tutorial exercises and interactive multi-media from our EARL product range.

Each course may be customised to you specific business and analytical requirements.

Each of these courses can be assessed if required

For more information on these courses or to download our 2007 training brochure – click here
 


Module 1 - Fundamentals of GC


  • Basic Principles of the Chromatographic Process
  • Overview of System Components
  • Main Mechanisms of Gas Chromatography (solute-stationary phase interactions)
  • Temperature / Retention Relationship
  • Sample Preparation Protocols
  • Sample Introduction - Principles, Choice of Technique, Operation (spilt / splitless, on-column)
  • Column Theory and Stationary Phase Considerations
  • Detectors - Choice and Operation (including FID, ECD, TCD, NPD)
  • Measuring and Optimising Chromatographic Parameters (R, N, a , k)
  • Basic Maintenance and Troubleshooting
  • Methods of Quantitation



Module 2 - GC Troubleshooting and Maintenance


(To include symptoms, causes, diagnosis and solution in each case)

  • Approaches to Troubleshooting - a discussion
  • Gas Delivery Systems
  • Hardware Troubleshooting and Maintenance (solvent, sample, temperature and pressure control, injection systems, column and detector systems)
  • Chromatographic Troubleshooting (based on chromatographic parameters and the chromatogram - baseline problems, peak shape, retention stability etc.)
  • Problems with quantitation
  • System records




Module 3 - GC Method Development


  • Establishing Method Objectives
  • Existing Information - what is known, what should be known
  • Chromatographic Theory Overview
  • Mechanisms of Separation
  • Equilibrium and the Distribution Constant
  • Solute - Stationary Phase Interaction
  • Sample Preparation Protocols
  • Injection System Choice - spilt / splitless, on-column, direct, PTV, headspace. (Each of the above will be considered with reference to the physical properties of the analyte / matrix / solvent which govern choice)
  • Carrier Gas Choice
  • Column Choice (stationary phase chemistry choice as governed by the analyte/s)
  • Detector System Choice / Operation, including FID, ECD, TCD, MS etc. (Once again, each of the above will be considered with reference to the physical properties of the analyte/matrix/solvent which govern choice)
  • Measuring and Optimising Chromatographic Parameters
  • Capacity Factor (k), Efficiency (N), Selectivity ( a ) and Resolution (R)
  • Temperature Effects
  • Appropriate Methods of Quantitation



Module 4 - Practical Exercises


These are available for inclusion in the above courses on request. Experiments are available to practically reinforce concepts that are covered in the various modules. The details of these can be arranged with the training sponsor to cover:

  • Column Installation and System Familiarisation
  • System Maintenance and Troubleshooting (detector, injector and gas delivery system troubleshooting)
  • Illustrating Effects of Stationary Phase Chemistry on Retention and Selectivity
  • Influence of Initial Oven Temperature on Resolution
  • Injection Optimisation Using Retention Gaps
  • Influence of Column Length, Diameter and Film Thickness on Retention Time and Efficiency
  • Separation Optimisation Using Temperature Programming
  • Injection Techniques - (filled needle, hot needle, solvent flush, split, splitless, on-column and autosampler)
  • Use of Internal Standards
  • Determination of Chromatographic Parameters (k, R, asymmetry, tailing, N and a )



 
  
 
 ©Crawford Scientific 2007