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Gas Chromatography

Fundamental Gas Chromatography
GC Troubleshooting & Maintenance
GC Method Development
Practical Exercises

Each of our Gas Chromatography courses are supplemented with full colour delegate handbooks as well as tutorial exercises and interactive multi-media from our earl product range.

Each course may be customised to your specific business and analytical requirements.

Each of these courses can be assessed if required

For more information on these courses or to download our 2008 training brochure – click here

Module 1 - Fundamentals of GC

Basic Principles of the Chromatographic Process
Overview of System Components
Main Mechanisms of Gas Chromatography (solute-stationary phase interactions)
Temperature / Retention Relationship
Sample Preparation Protocols
Sample Introduction - Principles, Choice of Technique, Operation (split / splitless, on-column)
Column Theory and Stationary Phase Considerations
Detectors - Choice and Operation (including FID, ECD, TCD, NPD)
Measuring and Optimising Chromatographic Parameters (R, N, a , k)
Basic Maintenance and Troubleshooting
Methods of Quantitation

Module 2 - GC Troubleshooting and Maintenance

(To include symptoms, causes, diagnosis and solution in each case)

Approaches to Troubleshooting - a discussion
Gas Delivery Systems
Hardware Troubleshooting and Maintenance (solvent, sample, temperature and pressure control, injection systems, column and detector systems)
Chromatographic Troubleshooting (based on chromatographic parameters and the chromatogram - baseline problems, peak shape, retention stability etc.)
Problems with quantitation
System records

Module 3 - GC Method Development

Establishing Method Objectives
Existing Information - what is known, what should be known
Chromatographic Theory Overview
Mechanisms of Separation
Equilibrium and the Distribution Constant
Solute - Stationary Phase Interaction
Sample Preparation Protocols
Injection System Choice - split / splitless, on-column, direct, PTV, headspace. (Each of the above will be considered with reference to the physical properties of the analyte / matrix / solvent which govern choice)
Carrier Gas Choice
Column Choice (stationary phase chemistry choice as governed by the analyte/s)
Detector System Choice / Operation, including FID, ECD, TCD, MS etc. (Once again, each of the above will be considered with reference to the physical properties of the analyte/matrix/solvent which govern choice)
Measuring and Optimising Chromatographic Parameters
Capacity Factor (k), Efficiency (N), Selectivity ( a ) and Resolution (R)
Temperature Effects
Appropriate Methods of Quantitation

Module 4 - Practical Exercises

These are available for inclusion in the above courses on request. Experiments are available to reinforce practically concepts that are covered in the various modules. The details of these can be arranged with the training sponsor to cover:

Column Installation and System Familiarisation
System Maintenance and Troubleshooting (detector, injector and gas delivery system troubleshooting)
Illustrating Effects of Stationary Phase Chemistry on Retention and Selectivity
Influence of Initial Oven Temperature on Resolution
Injection Optimisation Using Retention Gaps
Influence of Column Length, Diameter and Film Thickness on Retention Time and Efficiency
Separation Optimisation Using Temperature Programming
Injection Techniques - (filled needle, hot needle, solvent flush, split, splitless, on-column and autosampler)
Use of Internal Standards
Determination of Chromatographic Parameters (k, R, asymmetry, tailing, N and a )

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