Chromatography Consumables, Analytical Services and Chromatography / Mass Spectrometry Training from Crawford Scientific
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Liquid Chromatography - Mass Spectrometry




LC-MS for the Chromatographer
LC-MS Interpretation

 

Each of our LC-MS courses are supplemented with full colour delegate handbooks as well as tutorial exercises and interactive multi-media from our EARL product range.

Each course may be customised to you specific business and analytical requirements.

Each of these courses can be assessed if required

For more information on these courses or to download our 2008 training brochure – click here
 


LC-MS for the Chromatographer

This course is designed to cover the basic operating and theoretical principles of gas chromatography using mass selective detection. It will be most useful to those who have a working knowledge of GC-MS but wish to understand the principles that govern the technique.


Chromatographic Considerations


  • Basic Principles of the Chromatographic Process - Partition Theory
  • Solvent Delivery - Infusion Pumps / Syringe Pumps / Reciprocating Pumps /
  • Sample Introduction and Injection Valves / Isocratic and Gradient Elution.
  • Flow Rates and Flow Splitting
  • Choice of flow rate / Making a split / Concentration dependence
  • Commonly used solvents and buffers / Unsuitable solvents and buffers


LC- MS Hardware


  • Increasing tolerance of source / Hardware developments / Alternatives
  • Hardware Configurations for LC-MS - Maximising sensitivity and throughput /
  • Column Switching / Automated sample preparation
  • System Overview / Ion source development
  • The API Source - Electrospray / Atmospheric Pressure Chemical Ionisation / Routine Source Maintenance
  • Why use API / Mass Analyser - Quad, TOF, Ion Trap, Magnetic sector / Detector Systems.
  • The Mass Analyser - Quadrupole Instruments / Time of Flight Systems / Ion Traps / Magnetic Sector Instruments.


Instrument Tuning and Calibration, Applications & Designing MS Experiments


  • API Reference Materials / Solution Preparation and Introduction / Checking Calibration
  • Data Acquisition Modes - Full scanning / Selected Ion Recording
  • Quantitation - Internal standards / Analyte Considerations / Methods of Quantitation
  • Applications - Some useful worked examples of typical LC-MS applications
 



LC- MS Interpretation


This very popular course covers the principles of how to obtain good spectra – as well as having a host of tutor led exercises for crucial practice of interpretation skills and techniques.

  • Understanding isotope patterns and relative responses for confirmation of:
  • Elemental composition and chemical structure
  • Formation of common adduct ions under different mobile phase conditions
  • Compound dimerisation, multiply charged ions and the deconvolution process
  • Precursor ion collisionally induced dissociation (CID) fragmentation including:
  • Structural elucidation − The alpha-cleavage and inductive-cleavage
  • Target ion characterisation, peptide sequencing etc.
  • MS/MS scan function experiments − when to use them and how to interpret the results
  • Interpretation practice through consultant led tutorial exercises
  • ……and more!

 
  
 
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