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High Performance Liquid Chromatography



 Fundamental Liquid Chromatography
HPLC Troubleshooting & Maintenance
HPLC Method Development
2 day Method Development with Computer Simulation Exercises

Each of our Liquid Chromatography courses are supplemented with full colour delegate handbooks as well as tutorial exercises and interactive multi-media from our earlproduct range.

Each course may be customised to your specific business and analytical requirements.

Each of these courses can be assessed if required

For more information on these courses or to download our 2008 training brochure – click here
 

 Module 1 - Fundamentals of HPLC

  • Basic Principles of the Chromatographic Process
  • Overview of System Components
  • Main mechanisms of Chromatography and Principles of Ionisation
  • Sample Preparation Protocols
  • Sample Introduction - Principles, Choice of Technique and Operation
  • Column Theory and Stationary Phase Considerations
  • Detectors - Choice and Operation (including UV, RI, fluorescence)
  • Measuring and Optimising Chromatographic Parameters (R, N, a , k)
  • Basic Maintenance and Troubleshooting
  • Methods of Quantitation


 Module 2 - HPLC Troubleshooting and Maintenance


(To include symptoms, causes, diagnosis and solution in each case)

  • Approaches to Troubleshooting - a discussion
  • Hardware Troubleshooting and Maintenance (solvent, sample, temperature and pressure control, injection systems, column and detector systems)
  • Chromatographic Troubleshooting (based on chromatographic parameters and the chromatogram - baseline problems, peak shape, retention stability etc.)
  • Problems with quantitation
  • System records


 Module 3 - HPLC Method Development


All our method development courses are designed in conjunction with our clients to ensure maximum relevance. All include simulated method developments to highlight critical issues and concepts.

  • Strategies and Implementation Protocols
  • Existing Information - what is known, what should be known
  • Separation Processes
    adsorption chromatography, reverse phase, ion suppression, ion exchange, gel permeation, gel filtration, affinity chromatography
  • Sample Preparation Protocols
  • Choice and Optimisation of Detection Technique
  • Optimisation of Chromatographic Parameters - a practical approach
  • System Suitability Testing
  • Method Validation - an overview


 Module 4 – 2 day Method Development with Computer Simulation Exercises

 

These are available for inclusion in the above courses on request. Experiments are available to practically reinforce concepts that are covered in the various modules. The details of these can be arranged with the training sponsor to cover:


  • The Effect of pH on basic acidic and neutral species
  • Gradient optimisation, including Final %B and gradient Steepness
  • Use of the K* equation
  • Influence of Shape Selectivity
  • Calculation a suitable isocratic composition
  • Determination of Chromatographic Parameters
  • Column parameters; Particle size and flow rate effects
  • Treatment of the Van Deemter equation

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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