Chromatography Consumables, Analytical Services and Chromatography / Mass Spectrometry Training from Crawford Scientific
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High Performance Liquid Chromatography
 

 
  		Fundamental Liquid Chromatography
HPLC Troubleshooting & Maintenance
HPLC Method Development
2 day Method Development with Computer Simulation Exercises

Each of our Liquid Chromatography courses are supplemented with full colour delegate handbooks as well as tutorial exercises and interactive multi-media from our EARL product range.

Each course may be customised to you specific business and analytical requirements.

Each of these courses can be assessed if required

For more information on these courses or to download our 2007 training brochure – click here
 


Module 1 - Fundamentals of HPLC

  • Basic Principles of the Chromatographic Process
  • Overview of System Components
  • Main mechanisms of Chromatography and Principles of Ionisation
  • Sample Preparation Protocols
  • Sample Introduction - Principles, Choice of Technique and Operation
  • Column Theory and Stationary Phase Considerations
  • Detectors - Choice and Operation (including UV, RI, fluorescence)
  • Measuring and Optimising Chromatographic Parameters (R, N, a , k)
  • Basic Maintenance and Troubleshooting
  • Methods of Quantitation



Module 2 - HPLC Troubleshooting and Maintenance


(To include symptoms, causes, diagnosis and solution in each case)

  • Approaches to Troubleshooting - a discussion
  • Hardware Troubleshooting and Maintenance (solvent, sample, temperature and pressure control, injection systems, column and detector systems)
  • Chromatographic Troubleshooting (based on chromatographic parameters and the chromatogram - baseline problems, peak shape, retention stability etc.)
  • Problems with quantitation
  • System records



Module 3 - HPLC Method Development


All our method development courses are designed in conjunction with our clients to ensure maximum relevance. All include simulated method developments to highlight critical issues and concepts.

  • Strategies and Implementation Protocols
  • Existing Information - what is known, what should be known
  • Separation Processes
    adsorption chromatography, reverse phase, ion suppression, ion exchange, gel permeation, gel filtration, affinity chromatography
  • Sample Preparation Protocols
  • Choice and Optimisation of Detection Technique
  • Optimisation of Chromatographic Parameters - a practical approach
  • System Suitability Testing
  • Method Validation - an overview



Module 4 – 2 day Method Development with Computer Simulation Exercises


These are available for inclusion in the above courses on request. Experiments are available to practically reinforce concepts that are covered in the various modules. The details of these can be arranged with the training sponsor to cover:

  • Column Installation and System Familiarisation
  • System Maintenance and Troubleshooting (detector, injector and gas delivery system troubleshooting)
  • Illustrating Effects of Stationary Phase Chemistry on Retention and Selectivity
  • Influence of Initial Oven Temperature on Resolution
  • Injection Optimisation Using Retention Gaps
  • Influence of Column Length, Diameter and Film Thickness on Retention Time and Efficiency
  • Separation Optimisation Using Temperature Programming
  • Injection Techniques - (filled needle, hot needle, solvent flush, split, splitless, on-column and autosampler)
  • Use of Internal Standards
  • Determination of Chromatographic Parameters
  • (k, R, asymmetry, tailing, N and a )


 
  
 
 ©Crawford Scientific 2007