Run at our laboratory facilities in Manchester, these hands-on courses are designed to introduce both theory and practical concepts of instrumental analysis. Modern equipment is used in conjunction with interactive teaching techniques and well established practical exercises to ensure maximum return on your training investment.
These courses are invaluable for anyone wishing to have the most comprehensive grounding in instrumental analysis techniques or those wishing to increase their knowledge and practical ability with our advanced training programs.
There are only SIX places on each of these courses to allow maximum training impact – very early booking is advised as these courses are very popular indeed.
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Register – click here
Introduction to LC/MS |
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(practical exercises and experiments in italics)
- LC considerations for MS analysis including - Flow rates and Flow Splitting / Solvents, Buffers and Additives
- Instrument overview and optimisation
- ESI and APcI source interface overview and optimisation
- Nebuliser settings and gas pressures / temperatures
- In-source CID (Collision Induced Dissociation)
- LC-MS data acquisition modes.
- Scanning and SIM (Selected Ion Monitoring)
- Mass Analysers explained:
- Quadrupole
- Ion Trap
- Time of Flight
- LC-MS/MS data acquisition modes.
- Product Ion Scanning
- Precursor Ion Scanning
- Constant Neutral Loss
- Quantitative issues
- Optimising for sensitivity and specificity
- MRM (Multiple Reaction Monitoring) development
- Introduction to LC-MS Data and Spectral Interpretation
- Range of small molecule and biomolecule application and interpretation examples
- Common generic troubleshooting and maintenance issues
- Unknown Investigation Challenge!
Course Duration: 3 days
Course Hours: 9:00am – 5:00pm
Course Pre-requisites: A basic working knowledge of mass spec operation is helpful but this course can be also undertaken by complete novices with no prior hands-on experience
Travel & Accommodation: Located 10 mins. drive from Manchester Airport and local rail links. Enquire for further information on excellent rates with local hotels
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Practical HPLC Troubleshooting & Maintenance |
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(practical exercises and experiments in italics)
- Instrument problems – identifying the issue
- Common Maintenance Schedule Operations
- Maintenance of Pumps - pistons, mixers etc.
- Maintenance of Autosamplers – Rheodyne valves, seals, metering devices, syringes etc.
- Maintenance of detectors – Flow cell clean, Lamp replacement
- Common Calibration and Investigative Operations
- Log-keeping
- Calibrating Pump Flow
- Calibrating Autosampler volume
- Calibrating detector wavelength and response
- Testing Lamp Intensity
- Testing Flow Cell Cleanliness
- Calibrating Column Temperature
- Chromatographic Problem Solving
- Baseline problems
- Peak Shape Problems
- Retention Time Drift
- Peak Area Irreproducibility
- Putting it all together
- Commissioning an HPLC system from storage to tackle a particularly tricky separation!!
Course Duration: 2 days
Course Hours: 9:00am – 5:00pm
Course Pre-requisites: Experience with working with HPLC equipment is advantageous but not essential
Travel & Accommodation: Located 10 mins. drive from Manchester Airport and local rail links. Enquire for further information on excellent rates with local hotels
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Practical GC Troubleshooting & Maintenance |
(practical exercises and experiments in italics)
- Instrument problems – identifying the issue
- Common Maintenance Schedule Operations
- Importance of good quality gas supply
- GC Inlet Maintenance ( Split / Splitless) – including liners
- GC Detector Maintenance (FID practical) – also consider TCD / ECD / NPD
- Column Installation Conditioning and Storage
- Common Calibration and Investigative Operations
- Log-keeping
- Testing the inlet for leaks
- Establishing proper split flows - differences between split and splitless injection
- Establishing the hold-up time and calibrating the pressure control system and carrier gas flow
- Optimising detector settings
- Chromatographic Problem Solving
- Baseline problems
- Peak Shape Problems
- Retention Time Drift
- Peak Area Irreproducibility
- Putting it all together
- Commissioning a GC system from storage to tackle a particularly tricky separation!!
Course Duration: 2 days
Course Hours: 9:00am – 5:00pm
Course Pre-requisites: Experience with working with GC equipment is advantageous but not essential
Travel & Accommodation: Located 10 mins. drive from Manchester Airport and local rail links. Enquire for further information on excellent rates with local hotels
BOOK NOW
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HPLC Method Development |
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(practical exercises and experiments in italics use a mixture of HPLC experiments and simulations using LC optimisation software)
- Choosing columns – column dimensions, particle size, selectivity differences
- Optimising throughput using reduced column dimensions and particle size
- Optimising separation selectivity through stationary phase choice
- Using software to predict suitable columns
- Sample concentration, injection solvent, injection solvents
- Reverse phase HPLC:
- Solvent Choice
- Quick ways to optimise %B
- Analyte pH – influence of pKa and eluent pH on analyte retention
- Altering eluent pH to optimise separations
- Predicting and optimising separation robustness
- Choosing appropriate buffers and influence of buffer concentration
- Ion Pair Chromatography
- Influence of ion pair reagent type and concentration
- Gradient HPLC
- Characterising your system (dwell volume, re-equilibration volume etc.)
- Scouting Gradients
- Estimating Isocratic Conditions
- Calculating gradient conditions (starting and ending composition and gradient time)
- Bringing it all together
- Developing a method from scratch using all of the tools and tricks learnt on the course
Course Duration: 2 days
Course Hours: 9:00am – 5:00pm
Course Pre-requisites: Some experience of operating HPLC equipment and analysing HPLC data is required in order to fully participate in this course
Travel & Accommodation: Located 10 mins. drive from Manchester Airport and local rail links. Enquire for further information on excellent rates with local hotels
BOOK NOW
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Practical GC-MS
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(practical exercises and experiments in italics)
- GC Considerations for GC-MS
- Appropriate carrier gas and flow rate Column choices GC method parameters
- Ionisation in GC-MS – Electron Impact (EI) and Chemical (CI) Ionisation
- Ion Sources and Transfer Lines
- Ion source components and operating parameters Cleaning Ion Source Components
- Auto-tuning and manual tuning
- Interpreting tune reports Optimising the MS detector through manual tuning
- Quadrupole Mass Analysers - principles
- Experiment parameters – optimising SCAN and SIM experiment variables
- Ion Trap Mass Analysers – principles and typical data
- MS n – advantages and uses Data dependant scanning
- Detectors in GC-MS
- GC-MS Vacuum Systems
- Monitoring vacuum Establishing and breaking vacuum correctly Maintaining the vacuum system
- Bringing it all together
- Developing a GC-MS method for a particularly challenging determination
Course Duration: 2 days
Course Hours: 9:00am – 5:00pm
Course Pre-requisites: Some experience of operating GC-MS equipment and analysing GC-MS data is required in order to fully participate in this course
Travel & Accommodation: Located 10 mins. drive from Manchester Airport and local rail links. Enquire for further information on excellent rates with local hotels
BOOK NOW
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