YMC Overview : Stationary Phases

YMC’s product portfolio reflects the practical expertise in applying liquid chromatography for demanding separations, day after day - year after year, reproducibly and efficiently. The following chapter has been designed to outline a variety of chromatography tools that are found to be extremely useful in advanced working environments such as combinatorial chemistry, LC-MS, method development, validation and scale-up.

Analytical Stationary Phases Routinely Available from YMC

Feature highlights:


C30 carbon chain	high carbon load of 22%	large pH stability range	applicable for 100% aqueous eluents	high stability towards special chromatographic conditions

PRODUCT	CODE	PHASE (silica-based unless noted)	END CAPPED	USP CLASS NO.	PART. SIZE (µm)	PORE SIZE (nm)	CAR- BON LOAD (%C)	pH	TYPICAL APPLICATIONS
YMC Triart	TA	multi-layer hybrid particle	yes	L1	1.9, 3, 5	12	16 (20 total)	1.0-12.0	acid, neutral, basic and chelating compounds, metabolites, versatile stationary phase
YMC30	CT	proprietary polymeric bonding chemistry	pro-prietary	–	3, 51	pro-prietary	–	2-6	isomeric carotenes, retinols, steroids, fat-soluble vitamins
ProC18 & Ultra HT ProC18	AS	C18 using ultrapure silica base(99.999%), with very low residual non-specific interactions	yes	L1	2, 3, 51	12	16	2-8	fat-soluble vitamins, antioxidants, metabolites, acidic, neutral, basic and chelating compounds
ProC18RS	RS	high carbon load with polymeric bonding C18, ultrapure silica base (99,999%)	yes	L1	3, 5	8	22	1-10 [2]	suitable for a wide range of samples including acidic and basic compounds
Hydro-sphere C18 & Ultra HT Hydro-sphere C18	HS	based on the same ultrapure silica base as ProFamily, can be used in 100% aqueous eluent	yes	L1	2, 3, 5	12	12	2-8	strong polar compounds, antibiotics, catechol-amines, nucleic acids. water-soluble vitamins, acidic, neutral, basic and chelating compounds
ODS-A	AA	one of YMC’s international bestsellers, traditional high performance C18 column	yes	L1	3, 51	12, 20, 30	17, 12, 7	2-7.5	pharmaceuticals, vitamins, amino acids, peptides, general purpose phase
ODS-AM	AM	strict QC controlled production provides a high performance C18 column for validated methods operations	yes	L1	3, 51	12	17	2-7.5	purines, phenols, PTC-amino acids, angiotensins, alkaloids
ODS-AQ	AQ	"hydrophilic" endcapping, for 100% aqueous eluent systems, substantially increased retention of polar compounds	yes	L1	3, 51	12, 20	14, 10	2-7.5	strong polar compounds, pharmaceuticals, antibiotics, peptides and proteins, nucleic acids
J´sphere	JH, JM, JL	C18-family with differently controlled hydrophobicity for method development	yes	L1	4	8	22 JH 14 JM 9 JL	1-9 (H) 2-7.5 (M+L)	positional isomers, complexing agents, pharmaceuticals
ODS-AL	AL	traditional C18 for "mixed mode" separations	no	L1	3, 5	12	17	2-7.5	tocopherols, fat-soluble vitamins, disinfectants
Polymer C18	PC	polymethacrylate-matrix, stable towards shrinking and swelling, wide pH applicability	-	-	6, 101	pro-prietary	C18 equiv. 10%	2-13	phenols, anilines, peptides in high pH, pharmaceuticals, quaternary amines
ProC8	OS	latest generation C8 using ultrapure silica base (99.999%), with very low residual non-specific interactions	yes	L7	3, 5	12	10	2-7.5	acidic, neutral, basic and chelating compounds, drugs and metabolites
C8 (Octyl)	OC	traditional C8, high coverage monomeric bonding chemistry	yes	L7	3, 51	12, 20, 30	10, 7, 4	2-7.5	proteins and peptides, estrogens, general purpose phase
YMCbasic	BA	specifically designed for the separation of basic compounds	-	L7	3, 51	pro-prietary	8	2-7.5	basic molecules w/o modifiers, anilines, alkaloids, anti-depressants
Phenyl	PH	monomeric bonded phenyl	yes	L11	3, 51	12, 30	9, 3	2-7.5	phenols, fullerenes, sweeteners
ProC4	BS	latest generation C4 using ultrapure silica base (99.999%), with very low residual non-specific interactions	yes	L26	3, 5	12	7	2-7.5	polar acidic, neutral, basic and chelating compounds, polar peptides
C4 (Butyl)	BU	traditional C4, high coverage monomeric bonding chemistry	yes	L26	3, 51	12, 20, 30	7, 5, 3	2-7.5	biological separations, polar compounds
Protein RP	PR	specifically designed to withstand exposure to TFA, good recovery rates	yes	L26	5	pro-prietary		1.5-7.5	proteins, peptides
C1 (TMS) 3;4	TM	trimethyl silane, excellent hydrolytic stability	-	L13	3, 51	12, 30	4, 3	2-7.5	water-soluble vitamins
PVA-SIL	PV	polyvinyl alcohol bonded on silica support, suitable for normal or reversed phase applications	-	L24	5	12	-	2-9.5	proteins, phospho- lipids, retinoids, lipids
PolyamineII (PBMN) 4	PB	mixed secondary and tertiary amino derivative gives improved hydrolytic stability and alternative selectivity compared to conventional amino-phases, increased lifetime, rapid equilibration time	-	-	5	12	-	2-9	maltooligosaccha-rides, tocopherols, nucleotides, sugars
Amino 3;4	NH	primary amino derivative, high coverage monomeric bonding chemistry	-	L8	3, 51	12	3	2-7.5	sugars, nucleotides, water-soluble vitamins
Cyano 3;4	CN	traditional cyano derivative, useful also for SFC applications	yes	L10	3, 51	12, 30	7, 2.5	2-7.5	proteins, steroids, catechols
Diol 3;4	DL	for aqueous GPC or normal phase applications, high recovery of biological material	-	L20	51	6, 12, 20, 30	-	5-7.5 (RP) 2-7.5 (NP)	peptides, proteins, malto-oligo- saccharides
Silica 4	SL	ultra high purity, high mechanical stability	-	L3	3, 51	6, 12, 20, 30	-	2-7.5	small organic molecules, fat-soluble vitamins, tocopherols

1preparative grades routinely available (others on request).
2it is recommended to use at least 10% organic solvent composition near the pH limits and over 50% at pH values above pH 9.0 to preserve column lifetimes.
3when ordering please specify normal or reserved phase.
4applicable for HILIC-mode.