YMC-BioPro IEX Columns |
- newly developed porous hydrophilic polymer with low nonspecific adsorption
- excellent binding capacity and recovery of biomolecules
- very high resolution
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YMC-BioPro
QA-F |
YMC-BioPro
SP-F |
Matrix |
hydrophilic polymer bead |
hydrophilic polymer bead |
Pore size(nm) |
nonporous |
nonporous |
Particle size(μm) |
5 |
5 |
Charged group |
-CH2N+(CH3)3 |
-CH2CH2CH2SO3 |
Counter ion |
Cl- |
Na+ |
Ion-exchange capacity |
0.075-0.110 meq/ml-resin |
0.230-0.290 meq/ml-resin |
Dynamic binding capacity |
12-20 mg-BSA/ml-resin |
10-18 mg-human IgG/ml-resin |
Available pH range |
2.0 - 12.0 |
2.0 - 12.0 |
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| High efficiency with a lower column pressure |
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| Column |
Dimension |
Part Number |
| YMC-BioPro SP-F |
30x4.6 mm ID |
SF00S050346WP |
| YMC-BioPro QA-F |
30x4.6 mm ID |
QF00S050346WP |
| YMC-BioPro SP-F |
50x4.6 mm ID |
SF00S050546WP |
| YMC-BioPro QA-F |
50x4.6 mm ID |
QF00S050546WP |
| YMC-BioPro SP-F |
100x4.6 mm ID |
SF00S051046WP |
| YMC-BioPro QA-F |
100x4.6 mm ID |
QF00S051046WP |
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Comparison of column efficiency on non-porous polymer based columns*
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| Compared with the competitors’ columns, YMC-BioPro QA-F and SP-F show high theoretical plate counts, excellent peak shapes, and low pressure. Therefore, YMC-BioPro QA-F and SP-F is suitable for high-throughput analysis. |
| Eluent: |
20 mM Tris-HCl (pH 8.1) (for anion exchange columns)
20 mM KH2PO4-K2HPO4 (pH 6.8) (for cation exchange columns) |
| Flow rate: |
0.12 ml/min |
| Temperature: |
25 °C |
| Detection: |
UV at 220 nm |
| Injection: |
20 μl |
| Sample: |
Formamide (2 μl/ml) |
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High throughput analysis of proteins*
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YMC-BioPro SP-F can separate the proteins sharply without the peak-tailing observed in the chromatography of competitor’s SP-NPR. Furthermore, despite having a larger particle size, the theoretical plate number of YMCBioPro SP-F is higher.
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| Eluent: |
A) 20 mM KH2PO4-K2HPO4 (pH 6.8)
B) 20 mM KH2PO4-K2HPO4 (pH 6.8) containing 0.5M NaCl |
| Gradient: |
YMC-Bio SP-F 0-100% B (0-4 min)
TSKgel SP-NPR 0-100% B (0-4.67 min) |
| Flow rate: |
1.5 ml/min |
| Temperature: |
25 °C |
| Detection: |
UV at 220 nm |
| Injection: |
20 μl |
| Sample: |
1. Ribonuclease A (0.1 mg/ml)
2. Cytochrome c (0.1 mg/ml) |
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| Long column for high resolution analysis* |
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| The theoretical plate count for the 100 mm column is about 5 times higher than 30 mm column, making it very useful for high-resolution analyses. |
| Eluent: |
20 mM Tris-HCl (pH 8.1) |
| Flow rate: |
0.12 ml/min |
| Temperature: |
25 °C |
| Detection: |
UV at 220 nm |
| Injection: |
20 μl |
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Application - Nucleotides -*
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| Elution of DNA fragments is optimised on 100 mm columns. The sensitivity is improved by reducing the gradient to half. In addition, the analysis time can be shortened by increasing the buffer concentration, while maintaining excellent resolution. |
| Column: |
YMC-BioPro QA-F, 5 μm, 100 x 4.6 mm i.d. |
| Eluent: |
Eluent: A) 20 mM Tris HCl (pH 8.1) containing 0.5M NaCl
B) 20 mM Tris HCl (pH 8.1) containing 1.0M NaCl |
| Flow rate: |
0.5 ml/min |
| Temperature: |
25 °C |
| Detection: |
UV at 260 nm |
| Injection: |
20 μl |
| Sample: |
1 Kb DNA Ladder (0.25 mg/ml) |
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*By courtesy of YMC Co., Ltd.
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