YMC-BioPro IEX Columns |
- newly developed porous hydrophilic polymer with low nonspecific adsorption
- excellent binding capacity and recovery of biomolecules
- very high resolution
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YMC-BioPro
QA |
YMC-BioPro
SP |
Matrix |
hydrophilic polymer bead |
hydrophilic polymer bead |
Pore size(nm) |
100 |
100 |
Particle size(μm) |
5 |
5 |
Charged group |
-CH2N+(CH3)3 |
-CH2CH2CH2SO3 |
Counter ion |
Cl- |
Na+ |
Ion-exchange capacity |
0.075-0.100 meq/ml-resin |
0.070-0.095 meq/ml-resin |
Dynamic binding capacity |
110-150 mg-BSA/ml-resin |
70-100 mg-human IgG/ml-resin |
Available pH range |
2.0 - 12.0 |
2.0 - 12.0 |
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High binding capacity and high recovery
The porous type of YMC-BioPro shows great absorption capacity and excellent recovery, and is especially suitable for semipreparative separations of proteins and antibodies. |
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| Column |
Dimension |
Part Number |
| YMC-BioPro SP |
50x4.6 mm ID |
SPA0S050546WP |
| YMC-BioPro QA |
50x4.6 mm ID |
QAA0S050546WP |
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Comparison of dynamic binding capacity (DBC) for BSA
Compared with conventional porous polymer anion-exchange columns, YMC-BioPro QA gives
higher DBC and recovery rates. This indicates that YMC-BioPro has a much lower nonspecific
adsorption compared to conventional columns.
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Dynamic binding capacity
(mg/ml-gel, 10% breakthrough) |
Eluted amount
(mg/ml-gel) |
Recovery*
(%) |
| YMC-BioPro QA |
126 |
120 |
95 |
| Mono Q |
100 |
35 |
35 |
| BioAssist Q |
73 |
58 |
79 |
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| *Recovery: (Eluted amount/Dynamic binding capacity) x 100 |
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Before the determination, equilibrate the column with equilibration buffer.
Step 1: Load
A protein solution of known concentration is continuously loaded at the desired fl ow rate and the absorbance of the eluate is monitored until full saturation is achieved (100% UV absorbance of the pure sample solutions).
Step 2: Wash
Step 3: Elute
- Determination of DBC
The volume of protein solution that has been applied up to a specific breakthrough point (usually 5 or 10%) is a measure of the DBC of the medium.
High Mass Recovery
The high purity silica support and complete bonding coverage of Protein-RP results in very high sample recoveries. This is an important consideration when choosing a column for biochemical analyses. The table below shows recoveries of Proteins and Peptides. YMC-Pack Protein-RP provides higher recoveries than competitive C4 columns.
Superior resolution
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| Eluent: |
A) 20 mM KH2PO4-K2HPO4 (pH 6.8)
B) 20 mM KH2PO4-K2HPO4 (pH 6.8)
containing 0.5M NaCl
0-100% B (0-60 min., linear) |
1. Cytochrome c (0.5 mg/ml)
2. Ribonuclease A (0.5 mg/ml)
3. Lysozyme (0.5 mg/ml) |
| Flow rate: |
0.5 ml/min (4.6 mm i.d. column)
0.59 ml/min (5.0 mm i.d. column) |
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| Temperature: |
25 °C |
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| Detection: |
UV at 220 nm |
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| Injection: |
20 μl (4.6 mm i.d. column)
23.6 μl (5.0 mm i.d. column) |
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*By courtesy of YMC Co., Ltd.
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