YMC Analytical Columns: YMC-Pack C8 (Octyl)

General

YMC-Pack C8 is the most commonly used bonded phase after C18. Due to the moderate hydrophobicity; retention times tend to be shorter than those on ODS phases. YMC-Pack C8 is suitable for a wide range of sample types including pharmaceuticals and biologicals with a relatively high hydrophobicity.

YMC-Pack C8 packings are prepared by exhaustive bonding of a monomeric octylsilane to totally spherical and porous silica gel. The bonded phase is then treated with an exhaustive endcapping process to ensure a high surface coverage leading to a moderate 10% carbon loading on the standard 12 nm pore material. Compared to C18 phases, retention times for hydrophobic molecules will be shorter on C8 material due to the reduced carbon load.

YMC-Pack C8 is ideally suited for the separation of many compounds which are too strongly retained on C18 phases or require greater retention than provided by C4 materials. It is one of the most versatile reversed phase materials and should be considered for the development of new methods.

Available in three porosities, YMC-Pack C8 packings will separate many classes of compounds including pharmaceuticals, organic chemicals, peptides, protein and other biological molecules. For preparative applications, choose the smallest pore size which provides adequate retention and resolution. This is because sample loading is generally proportional to surface area. Smaller pore packings provide greater surface area.

YMC-Pack C8 (Octyl) is stable towards hydrolysis between pH 2-7,5. Remove acid and buffer salts before storage. Store the column in methanol / water = 70/30.

For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column.