YMC Analytical Columns: YMC-Pack Diol

good selectivity without excessive retention
high product recovery rate
high prep loading
bonded phase reproducibility
improved peak shape versus bare
gel filtration on a silica based material for aqueous size separations

Click here to view the full list of YMC-Pack Diol parts

YMC-Pack Diol	Specification	Specification	Specification	Specification
Particle size / µm	5	5	5	5
Pore size / nm	6	12	20	30
Surface area / m2g-1	-	330	175	100
Carbon content / %	5.0 - 7.5	5.0 - 7.5	5.0 - 7.5	5.0 - 7.5
Recommended pH range	2.0 - 7.5	2.0 - 7.5	2.0 - 7.5	2.0 - 7.5

General

In normal phase mode the YMC-Pack Diol stationary phase is a versatile alternative to silica. The bonded phase’s hydroxyl groups provide good selectivity without excessive retention, since hydrogen bonding with the diol layer is not as strong as with the silanols on a bare silica surface. Diol columns also provide improved reproducibility when compared with bare silica.
Diol packings are suitable for separations using reversed phase techniques or molecular weight determination of proteins by gel filtration.

Properties

As with all YMC silica based bonded phases, YMC-Pack Diol starts with a base silica support of exceptional purity. YMC manufacturing and quality control procedures ensure that the silica has a very low residual metal content. The silica purity greatly reduces non-specific sample adsorption, thereby providing excellent sample recovery.

The high surface area, together with the large number of available sites for interaction of the 1,2-dihydroxypropane ligands, provides high preparative loading.

YMC-Pack Diol GPC columns exhibit better performance characteristics than underivatised silica for size separations as the non-specific adsorptive sites have been eliminated. Diol is available in four porosities: 6, 12, 20 and 30 nm and thus it is suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.

YMC-Pack Diol packings can be cleaned repeatedly with methanol, or even water. When combined with the high mechanical strength of the pure base silica, this washability means that YMC-Pack Diol packings provide longer column life than underivatised silica.

Column care

YMC-Pack Diol is stable towards hydrolysis between pH 5-7,5 in RP mode and pH 2-7,5 in NP mode. Remove acid and buffer salts before storage. For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column.

YMC-Pack Diol

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