YMC Analytical Columns: YMC-Pack ODS-AL |
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possesses residual silanols for mixed-mode separations
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same high ligand density as other YMC ODS phases
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unique selectivity for polar compounds
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Click here to view the full list of ODS-AL parts |
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Specification |
Particle size / µm |
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Pore size / nm |
12 |
Surface area / m2g-1 |
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Carbon content / % |
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Recommended pH range |
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Non-endcapped ODS
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YMC-Pack ODS-AL uses not only hydrophobic interaction but also secondary interactions with reactive residual silanol groups to affect separation. This results in a different selectivity from conventional ODS columns. When ionic interactions are involved, it is preferable to use a buffer in the mobile phase to achieve reproducibility of chromatograms. |
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1. Uracil
2. Pyridine
3. 2,6-diemetylpyridine
4. Methylparaben |
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Column : YMC-Pack ODS-AL and ODS-AQ (5µm, 12nm) 150 x 4.6mm ID
Eluent : 100mM KH2PO4 – Na2HPO4 (pH 7.0) /methanol (75/25)
Flow rate : 0.8 ml/min
Temperature : 30°C
Detection : UV at 270nm
(Condition described in Japanese Pharmacopoeia 12th ed.) |
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Utilises residual silanol groups for separat
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| The figure above shows separation of microbiocides under the conditions described in the Japaneses Pharmacopeia. The object is to select the column which allows the elution of benzoic acid, salicylic acid and theophylline, in this order, with complete separation of these peaks. With ODS-AQ, separation of salicylic acid and theophylline is incomplete. By contrast ODS-AL provides excellent separation. Therefore ODS-AL may provide excellent separation when the separation conditions cannot be optimised on other ODS columns. |
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| Column care |
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YMC-Pack ODS-AL is stable towards hydrolysis between pH 2-7,5. Remove acid and buffer salts before storage. Store the column in methanol / water = 70/30.
For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column. |
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