YMC Analytical Columns: YMC-Pack ODS-AQ |
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- balanced polarity for:
- pharmaceuticals
- antibiotics
- peptides and proteins
- nucleic acids
- oligosaccharides
- water-soluble vitamins
- carboxylic acids
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Click here to view the full list of ODS-AQ parts
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Specification |
Specification |
Particle size / µm |
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3;5 |
Pore size / nm |
12 |
20 |
Surface area / m2g-1 |
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175 |
Carbon content / % |
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10 |
Recommended pH range |
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2.0 - 7.5 |
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General |
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YMC-Pack ODS-AQ is a C18 reversed phase silica based HPLC packing material specifically designed for use in 100% aqueous eluents. As a result of the proprietary derivatisation process, YMC-Pack ODS-AQ exhibits a different selectivity to that of traditional C18 stationary phases. This difference in selectivity of YMC-Pack ODS-AQ, can be used to advantage for HPLC separations which are difficult to achieve with conventional C18 columns. |
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Selectivity Data
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The proprietary YMC derivatisation process creates the different selectivity of YMC-Pack ODS-AQ, where:
- The activity of acidic unreacted silanols is reduced, allowing moderately basic compounds to be eluted with little or no peak tailing.
- The balanced hydrophilic/lipophilic nature of the YMC-Pack ODS-AQ stationary phase leads to strong retentions of polar sample solutes even in aqueous eluents.
These properties of YMC-Pack ODS-AQ are beneficial for separations of polar
organic compounds, which tend not to be retained or are unresolved when
conventional C18 columns are used.
Many conventional ODS packings lose their ability to retain polar compounds in these high aqueous content mobile phases as shown below. They appear less lipophilic with collapsed C18 chains. However, in similar mobile phases, YMC-Pack ODS-AQ maintains its brush-like C18 chain structure and its lipophilic properties and provides excellent retention of polar compounds. |
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| Applications |
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YMC-Pack ODS-AQ is able to resolve compounds with minor differences in polarity from closely related chemical structures. As a result, YMC-Pack ODS-AQ is an excellent tool for the separation of drugs and corresponding metabolites, pesticides and degradation products, or peptides and protein digests etc. This capability of “polar recognition” opens up a broad application range for YMC-Pack ODS-AQ in life sciences and pharmacology.
Genuine linear scale-up from analytical to large scale separations is easily achievable with YMC products such as YMC-Pack ODS-AQ, where particle sizes from 3 to 50 µm are available in large lot sizes up to several hundred kilograms, if needed. This, together with the outstanding selectivity of YMC-Pack ODS-AQ, makes it an essential tool to enhance the productivity of large scale chromatographic processes. |
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| Comparison of ODS-AQ vs. Conventional ODS |
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Conventional ODS in
standard organic mobile phases |
Conventional ODS in
100% aqueous mobile phases |
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Column: YMC-Gel ODS-A
Dimension: 250 x 10 mm
Particle size: 25 μm
Mobile Phase: 100% water
Compounds: Highly polar and very water soluble |
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YMC-Gel ODS-AQ in
standard organic mobile phases
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YMC-Gel ODS-AQ in
100% aqueous mobile phases
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Column: YMC-Gel ODS-AQ
Dimension: 250 x 10 mm
Particle Size: 25 μm
Mobile Phase: 100% water
Compounds: Highly polar and very water soluble |
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| Column care |
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| The recommended pH range for YMC-Pack ODS-AQ is 2-7,5 in up to 100% aqueous systems and a maximum of 50 °C. Remove acid and buffer salts before storage. Store the column in methanol / water = 70/30. Clogged inlet frits often can be cleaned by changing the flow direction or replacement. For detailed information please refer to the “Column Care and Use Instructions”, which are shipped with each analytical column. |
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