YMC-Gel Polymer C18 is a reversed phase liquid chromatography packing which provides a broad range of solvent choices and a pH range from 2 – 13. YMC-Gel Polymer C18 is manufactured from a hydrophilic methacrylate polymer which is cross-linked with C18 ligand-containing reagents. YMC-Gel Polymer C18 offers a maximum application range: A wide variety of compounds such as organic acids, organic amines, peptides, pharmaceuticals and proteins can be separated using YMC-Gel Polymer C18.
YMC-Gel Polymer C18 is prepared from a hydrophilic methacrylate polymer bonded with a hydrophobic octadecylsilane reagent to make the C18 functionality an integral part of the polymeric structure.
Polymer C18 is a three-dimensional polymer matrix which is not based on a silica gel support.
As such, it has no residual silanols or metal impurities to interfere with the separation of basic organic compounds.
YMC-Gel Polymer C18 is compatible with all common reversed phase eluents such as water, methanol, acetonitrile and THF. Virtually all aqueous buffers and acid modifiers, such as TFA and phosphoric acid, as well as base modifiers such as sodium hydroxide and ammonium hydroxide can be used. Since it resists shrinking and swelling, YMC-Gel Polymer C18 can be used with eluents ranging in composition from 100 % aqueous to 100 % organic component.
In addition, YMC-Gel Polymer C18 can easily be sterilised by flushing with 0.1N NaOH in 20 % acetonitrile/water.
The selectivity and retention of YMC-Gel Polymer C18 is similar to standard ODS phases, due to its hydrophobic bonding on a hydrophilic support. Consequently, its selectivity is closer to that of silica-based C18 supports than to styrene/DVB-based supports.
It should be noted that interactions between aromatic or conjugated systems and the methacrylate backbone provides slightly greater retention when compared to silica-based ODS columns, whereas highly aliphatic compounds show greater retention on silica-based ODS supports.