YMC Analytical Columns: The Pro Family |
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- YMC-Pack Pro C18, Pro C8, Pro C4 based on ultra high purity silica
- UltraHT Pro C18 & UltraHT Hydrosphere for Fast-LC (2µm particle size)
- YMC-Pack Pro C18 RS; as a high carbon load version (C= 22%)
- Hydrosphere C18 for stability in aqueous mobile phases
- Every packed column supplied with:
lot certificate on physicochemical properties of bare silica, minimal metal traces, bonding and chromatographic performance
- Test chromatogram showing high performance plate count together with tight specification on peak symmetry
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Particle size / µm |
2**;3;5 |
3;5 |
3;5 |
3;5 |
2**;3;5 |
Pore size / nm |
12 |
12 |
12 |
8 |
12 |
Surface area / m2g-1 |
340 |
340 |
340 |
510 |
340 |
Carbon content / % |
16 |
10 |
7 |
22 |
12 |
Recommended pH range |
2-8 |
2-7.5 |
2-7.5 |
1-10*** |
2-8 |
Specification metal content |
Actual* |
Actual* |
Actual* |
Actual* |
Actual* |
Al (ppm) < 10 |
0.3 |
0.2 |
0.6 |
0.3 |
0.7 |
Fe (ppm) < 10 |
2.8 |
2.5 |
2.9 |
0.1 |
1.2 |
Na (ppm) < 10 |
0.3 |
1.4 |
1.0 |
1.3 |
0.7 |
Ti (ppm) < 10 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
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*randomly selected lots
**UltraHT for Fast-LC
***it is recommended to use at least 10% organic solvent composition near the pH limits and over 50% at pH values above pH 9.0 to preserve column lifetimes |
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General
One of the main challenges in RP-HPLC is the quantitation of ionisable compounds
including drugs, degradation products, etc. For this purpose symmetrical, sharp
peaks are required to provide highest resolution and reliable integration. The
stationary phases of the YMC ProFamily fulfil these demands making them an
excellent choice for the pharmaceutical and biotechnology industries. This
product line consists of the three octadecyl phases YMC-Pack Pro C18 RS,
YMC-Pack Pro C18 and Hydrosphere C18 together with the octyl- and butyl-phase
YMC-Pack Pro C8 and YMC-Pack Pro C4.
Properties
The base material for the stationary phases of the ProFamily is silica, which is covered with silanol groups on the surface. In the derivatisation step, these groups are reacted with, for example, octadecylsilanes to give C18 reversed phases. To minimize unwanted interactions between residual silanols and the sample molecules that are responsible for asymmetric peaks, YMC utilises a proprietary, highly effective endcapping process.
In addition, silica with an extremely low metal content is used for the phases of the ProFamily to suppress polar interactions, as metal impurities influence silanol groups to become more acidic. The table above illustrates that the total metal content is lower than 10 ppm for those elements reported |
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Lot-to-lot reproducibility for Pro C18, 5 µm
In this comparison, phenol and pyridine were selected to
show the peak performance for acidic and basic compounds, respectively. The low metal content and the proprietary endcapping result in a very good peak performance from
lot-to-lot-to-lot.
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Hydrophobicity and steric selectivity
This comparison shows the different properties of the ProFamily members giving a good indication on their potential for method development.
The compounds 1. uracil (dead volume marker) 2. n-butylbenzene 3. o-terphenyl 4. n-amylbenzene and 5. triphenylene are used to determine the hydrophobicity (2 and 4) and the steric selectivity (3 and 5) of each ProFamily member under non-buffered chromatographic conditions. |
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YMC-Pack Pro C18
Medium hydrophobicity and steric
selectivity  good baseline separation
of all
compounds
Column: 150 x 4.6 mm i.d.
Eluent: methanol / water (80/20)
Flow rate: 1.0 ml/min
Temperature: 37° C
Detection: UV at 254 nm
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YMC-Pack Pro C8
Lower hydrophobicity shorter retention
times
Lower steric selectivity close elution of
compound 3 and 5
Column: 150 x 4.6 mm i.d.
Eluent: methanol / water (80/20)
Flow rate: 1.0 ml/min
Temperature: 37° C
Detection: UV at 254 nm
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YMC-Pack Pro C4
Lowest hydrophobicity in this comparison
very short retention times
Short C4 chains less steric selectivity
Column: 150 x 4.6 mm i.d.
Eluent: methanol / water (80/20)
Flow rate: 1.0 ml/min
Temperature: 37° C
Detection: UV at 254 nm
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YMC-Pack Pro C18 RS
Highest carbon load highest hydrophobicity
increased retention times
High steric selectivity late elution for triphenylene
Column: 150 x 4.6 mm i.d.
Eluent: methanol / water (80/20)
Flow rate: 1.0 ml/min
Temperature: 37° C
Detection: UV at 254 nm
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Hydrosphere C18
Designed for pure aqueous eluents and polar compounds under the present conditions chromatographic behaviour between C8 and C18
Column: 150 x 4.6 mm i.d.
Eluent: methanol / water (80/20)
Flow rate: 1.0 ml/min
Temperature: 37° C
Detection: UV at 254 nm
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The whole ProFamily covers a large area of hydrophobicity and steric selectivity, as presented in this comparison, which offers the opportunity to accomplish optimisation of chromatographic methods even for complicated separation problems. |
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