YMC Analytical Columns: Pro Family : YMC-Pack Pro C18 |
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- specifically designed for pharmaceutical and biotechnology R&D
- UltraHT Pro C18 for Fast-LC (2µm particle size)
- extremely narrow specifications
- high lot-to-lot reproducibility
- high column-to-column reproducibility
- ideal for basic, acidic and polar compounds
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Click here to view the full list of Pro C18 parts
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Specification |
Particle size / µm |
2*;3;5 |
Pore size / nm |
12 |
Surface area / m2g-1 |
340 |
Carbon content / % |
16 |
Recommended pH range |
2 - 8 |
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| *UltraHT for Fast-LC |
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General
In 1996, YMC introduced the first member of the ProFamily, YMC-Pack Pro C18, into the market. Since then, YMCPack Pro C18 has proved to be one of the first choices for a wide range of HPLC applications in pharmaceutical and biotechnological research and production, where efficiency and reliability are highly appreciated.
The main reasons for this success are, besides the very good performance in HPLC separations, the high lot-to-lot and column-to-column reproducibility which are essential for chromatography in general. The achievement of this superior performance is not as trivial as first impressions might give. To ensure high quality continuously, a consistent and stringent controlling system is required. The figure on the right demonstrates the high lot-to-lot reproducibility for three lots selected at random. The stringent specifications applied as HPLC Test A, and the actual results obtained from the particular lot, are displayed on each lot inspection report, which is supplied with each individual column.
The retention of compounds ethylbenzene and butoxybenzoic acid relative to propylbenzene is used to monitor the hydrophobicity of each individual lot.
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| Lot-to-lot reproducibility of YMC-Pack Pro C18 |
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HPLC Test A
Column: 150 x 4.6 mm i.d.
Eluent: 20 mM KH2PO4-H3PO4 (pH3.5)/acetonitrile (40/60,v/v)
Flow rate: 1.0 mL/min
Detection: UV at 254nm
Temperature: 37 °C |
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YMC-Pack Pro C18 is also available in 10 particle size for preparative applications, in bulk and prepacked columns.
High lot-to-lot reproducibility is the essential basis for high performance HPLC columns. A high column performance itself can only be achieved when the columns are well packed and continuously give high numbers of theoretical plates and good asymmetry factors. The figure below clearly demonstrates the high level of reproducible quality from column to column to column.
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| Column-to-column comparison for consecutive packed columns |
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Column: 150 x 4.6 mm, 5 µm
Eluent: acetonitrile / water (60/40)
Flow rate: 1.0 ml/min
Detection: UV at 270 nm, 0.32 AUFS
Temperature: 25 ºC |
Plate count and asymmetry were calculated for butyl benzoate.
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Properties
YMC-Pack Pro C18 is based on an ultra pure silica support, which is used for the whole ProFamily. Due to a proprietary endcapping process especially designed for this type of silica, YMC-Pack Pro C18 is perfectly suitable for the separation of acidic and basic molecules. The inertness of the silica makes it an excellent choice for the analysis of drugs or metabolites, compounds that are susceptible to polar interactions with residual silanol groups and metal impurities as demonstrated in the following comparison.
The extreme basic substances are selected to prove the very good performance of YMC-Pack Pro
C18 in regard to their separation and the peak performance that cannot be achieved with classical
materials.
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| Separation of extremely basic compounds |
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Tricyclic antidepressants
Column: 150 x 4.6 mm i.d.
Eluent: 20 mM potassium phosphate buffer (pH 6.9) / methanol (25/75)
Flow rate: 1.0 ml/min
Temperature: 37°C
Detection: UV at 254 nm |
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Column care
YMC Pack Pro C18 is stable towards hydrolysis between pH 2-8. Remove acid and buffer salts before storage. Store the column in methanol / water = 70/30.
For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column. |
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