The relatively high carbon load of YMC-Pack Pro C18 RS amplfies the columns ability to selectively discriminate between closely related compounds such as positional or steric isomers.
A good system to test is a mixture of o-, m- and p-terphenyl as these compounds differ only in their three dimensional structure and not their hydrophobicity or polarity. YMC-Pack Pro C18 RS recognises even slight steric differences, unlike a conventional C18 (15% carbon load) as shown in the chromatogram below. |