YMC Analytical Columns: Pro Family : YMC-Pack Pro C8 |
| |
- extremely broad selectivity pattern
- good alternative to C18-phases
- excellent stability in acidic and alkaline eluents
- suitable for all types of organic molecules, especially basic pharmaceuticals
|
Click here to view the full list of YMC-Pack Pro C8 parts
|
|
Specification |
Particle size / µm |
|
Pore size / nm |
12 |
Surface area / m2g-1 |
340 |
Carbon content / % |
10 |
Recommended pH range |
|
|
| |
General
Within the ProFamily, the YMC-Pack Pro C8 provides an additional, less hydrophobic stationary phase for all types of compounds, but especially for basic and metal chelating substances. For many applications regarding the separation of peptides, nucleic acids and similar compounds with LC-MS detection, conventional C8-stationary phases require ion pair reagents and ion-suppression to obtain satisfactory separation and low detection limits. In contrast, Pro C8 with its ultra pure silica allows the analysis without these modifiers but still generates excellent chromatograms. In addition to the reduced hydrophobicity of YMC-Pack Pro C8 compared with YMC-Pack Pro C18, the different steric selectivity offers new possibilities in method optimisation as demonstrated in the figure below: |
|
| |
|
| |
| Steric selectivity YMC-Pack Pro C18 vs. YMC-Pack Pro C8 |
 |
Column: 150 x 4.6 mm i.d.
Eluent: methanol / water (80/20)
Flow rate: 1.0 ml/min
Temperature: 37° C
Detection: UV at 254 nm |
|
| |
|
| |
YMC-Pack Pro C8 is a member of the ProFamily and as a result gives excellent peak shapes even for basic substances, due to its low metal content and the endcapping procedure, which is identical to that used for YMC-Pack Pro C18. This shall be demonstrated in the comparison below where YMC-Pack Pro C8 outperforms competitive state of the art products.
|
| |
| Peak performance of YMC-Pack Pro C8 for basic substances* |
 |
Histamine antagonists
Column: 150 x 4.6 mm i.d.
Eluent: 20mM KH2PO4-K2HPO4 (pH 6.9) / methanol (35/65)
Flow rate: 1.0 ml/min
Temperature: 37° C
Detection: UV at 260 nm, 0.26 AUFS |
*By courtesy of YMC Co., Ltd. |
| |
|
| |
Column care
YMC-Pack Pro C8 is stable towards hydrolysis between pH 2-7,5. Remove acid and
buffer salts before storage. Store the column in methanol / water =
70/30.
For detailed information please refer to the “Column Care and Use Instructions” which are shipped with each analytical column. |
| |
